Browsing Theses by Subject "fluorescence"
Now showing items 1-18 of 18
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Automatic Identification of Algae using Low-cost Multispectral Fluorescence Digital Microscopy, Hierarchical Classification & Deep Learning
(University of Waterloo, 2019-12-05)Harmful algae blooms (HABs) can produce lethal toxins and are a rising global concern. In response to this threat, many organizations are monitoring algae populations to determine if a water body might be contaminated. ... -
Characterization of PIBSI Dispersants and Their PIBSA Components by Pyrene Excimer Fluorescence
(University of Waterloo, 2020-12-18)Methods are introduced to characterize the products resulting from the coupling of a polyisobutylene terminated at one end with a succinic anhydride (PIBSA) and a polyamine to generate PIBSI products where the polyamine ... -
Characterization of Polysaccharides in Starch using Fluorescence Techniques
(University of Waterloo, 2020-09-25)Amylose and amylopectin, which are the two main constituents of starch, were characterized using the pyrene excimer fluorescence (PEF) technique. First, an amylose sample was randomly labeled with the dye pyrene to yield ... -
Characterization of the Behaviour of Solution-Responsive Polymers by Fluorescence
(University of Waterloo, 2014-04-17)Two families of amphiphilic polymers were characterized with respect to their ability to respond to changing solution conditions using steady-state and time-resolved fluorescence. Firstly, a series of 5 sequential amphiphilic ... -
Characterization of the Conformation and Internal Backbone Dynamics of a-Polyglucans by Pyrene Excimer Fluorescence
(University of Waterloo, 2021-11-23)The conformation and internal backbone dynamics (IBD) of several pyrene-labeled polysaccharides (Py-PSs), namely Py-Amylopectin, Py-Glycogen, Py-Dextran, and Py-Pullulan, were successfully examined with a combination of ... -
Characterization of the Self-Assembly of Pyrene-Labelled Macromolecules in Water
(University of Waterloo, 2010-05-19)The self-assembly of several pyrene-labelled amphiphilic macromolecules in water was characterized by fluorescence. Information on their self-assembly was obtained by monitoring the level of pyrene aggregation in solution. ... -
Characterizing Polymers with Complex Architecture using Pyrene Excimer Fluorescence
(University of Waterloo, 2021-09-29)This thesis combines two different models, namely the model free analysis (MFA) and the fluorescence blob model (FBM), that were used to study the conformation and internal dynamics of two highly branched macromolecules, ... -
Daptomycin: studies on its action mode and on bacterial resistance with model membranes
(University of Waterloo, 2016-05-16)Bacterial resistance to antibiotics is one of the most serious problems facing medicine today. Recently, reports have appeared describing bacteria that are resistant to daptomycin (dap), an important antibiotic used against ... -
Fluorescence and Adaptation of Color Images
(University of Waterloo, 2011-04-26)Color plays a vitally important role in the world we live in. It surrounds us everywhere we go. Achromatic life, restricted to black, white and grey, is extremely dull. Color fascinates artists, for it adds enormously ... -
Fluorescently Labeled Latex Particles to Monitor Film Formation
(University of Waterloo, 2017-10-25)The influence of the length of an oligo(ethylene glycol) spacer (EGn, where n is the number of ethylene glycol units) in a 1-pyrenemethoxy-EGn methacrylate (PyEG-MA) monomer on the incorporation of the monomer into a ... -
Interfacing fluorescent DNA oligonucleotides with graphene oxide and metal oxides: from adsorption to sensing
(University of Waterloo, 2022-08-18)DNA, apart from being the mode of genomic information storage, has found several uses in catalysis (DNAzymes) and target detection (aptamers). Developing novel biosensors utilizing these properties has therefore been a ... -
Pyrene Excimer Formation: A Tool to Study Macromolecular Conformations in Solution
(University of Waterloo, 2024-04-26)The current view for fluorescence collisional quenching (FCQ) experiments is that no quantitative information can be retrieved from macromolecules containing more than a single dye-quencher pair attached at two specific ... -
Quantitative Characterization of Pyrene-Labeled Macromolecules in Solution by Global Analysis of Fluorescence Decays
(University of Waterloo, 2012-04-27)A series of pyrene end-labeled monodisperse poly(ethylene oxide)s (PEO(X)-Py2 where X represents the number average molecular weight (Mn) of the PEOs and equals 2, 5, 10 and 16.5 K) and one pyrene mono-labeled PEO (PEO(2K)-Py1) ... -
Selection and characterization of an aptamer to a thiazole orange derivative for use in a fluorescence-based biosensor
(University of Waterloo, 2023-08-18)Aptamers are short single-stranded DNA or RNA sequences that have been selected in vitro to have a high binding affinity to a specific target molecule. One class of aptamers can bind to small non-fluorescent molecules to ... -
Study of Arborescent Poly(L-Glutamic Acid) by Pyrene Excimer Formation
(University of Waterloo, 2012-08-31)The biological function of a protein is determined by its amino acid sequence, structure, and internal dynamics. In turn the prediction of a protein structure from its folding pathway involves the characterization of the ... -
Synthesis and Characterization of Two Pyrene Labeled Gemini Surfactants and their Interactions with Oppositely Charged Molecules
(University of Waterloo, 2022-06-09)Cationic gemini surfactants (GS) have attracted the attention of the scientific community as potential carriers of genetic cargo for gene transfection applications. These GS/polynucleotides complexes have been extensively ... -
Synthesis and Study of the Cyclic Lipopeptide Antibiotics Paenibacterin and Daptomycin
(University of Waterloo, 2021-09-23)Cyclic lipopeptides present a rich source of antimicrobial compounds with diverse modes of action. Paenibacterin is a cyclic lipopeptide antibiotic produced by Paenibacillus thaminolyticus that exhibits activity against a ... -
Using Pyrene to Probe Interparticle Polymer Diffusion in a Latex Film
(University of Waterloo, 2015-12-16)Fluorescence has been used for many years as a powerful analytical tool to probe the formation of films from aqueous latex dispersions. Currently, the most widely used method is fluorescence resonance energy transfer (FRET), ...