Browsing Theses by Subject "Chemistry"
Now showing items 21-40 of 266
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Biochemical and Biophysical Investigations of Non-Zinc Dependent Glyoxalase I Enzymes
(University of Waterloo, 2008-09-26)The principal methylglyoxal (MG)-detoxifying system in most living organisms is the two metalloenzyme Glyoxalase system. Glyoxalase I (GlxI) initially converts the non-enzymatically formed MG-GSH hemithioacetal to the ... -
Biochemical Investigations of L-Methionine gamma-lyase 1 from Trichomonas vaginalis
(University of Waterloo, 2011-12-16)The enzyme L-methionine γ-lyase (MGL) utilizes a pyridoxal-5’-phosphate-cofactor in order to convert L-methionine to α-ketobutyrate, ammonia and methyl mercaptan. MGL is proposed to be a potential drug target since it is ... -
Biochemistry in Bacterioferritin
(University of Waterloo, 2006)Bacterioferritin, an iron storage protein having a 24-subunit quaternary structure, was used as a model for the study of host-guest interactions and guest encapsulation, making use of its spherical cage-like structure. ... -
BIOCOMPATIBLE SOLID PHASE MICROEXTRACTION
(University of Waterloo, 2006-12-12)Today’s solid phase microextraction (SPME) is a well known technique that combines knowledge from different fields in an attractive, efficient, and economic way. The development of SPME has seen huge growth since its ... -
Biosensing at an individually addressable electrochemical array
(University of Waterloo, 2006)In this thesis, a novel electrochemical array is reported. The array consists of two planar halves, each having four carbon screen-printed band electrodes (SPEs), orthogonally facing each other and separated by a spacer ... -
Calmodulin as a universal regulator of voltage gated calcium channels
(University of Waterloo, 2014-05-22)Calmodulin (CaM) is a ubiquitous calcium-binding protein responsible for the binding and activation of a vast number of enzymes and signaling pathways. It contains two lobes that bind two calcium ions each, separated by a ... -
Calmodulin Binding and Activation of Mammalian Nitric Oxide Synthases
(University of Waterloo, 2008-05-12)Calmodulin (CaM) is a ubiquitous cytosolic Ca2+-binding protein involved in the binding and regulation of more than three-hundred intracellular target proteins. CaM consists of two globular domains joined by a central ... -
Carbon Nanotubes as Versatile Devices for Detoxification and Cellular Entry
(University of Waterloo, 2012-10-30)The ability to bypass most cellular barriers to gain access to intracellular compartments has great potential in cell biology. The possibilities range from efficient delivery of macromolecules such as plasmids to small ... -
The Catalytic Intramolecular Friedel-Crafts Acylation of Meldrum's Acid Derivatives and The Total Synthesis of Taiwaniaquinol B
(University of Waterloo, 2005)The intramolecular Friedel-Crafts acylation of aromatics with Meldrum?s acid derivatives catalyzed by metal trifluoromethanesulfonates and other Lewis acids is reported. Meldrum?s acids are easily prepared, functionalized, ... -
Catalytic Potential and Ligand Binding Properties of the Malachite Green RNA Aptamer
(University of Waterloo, 2008-09-18)The malachite green aptamer was originally engineered for binding specificity to malachite green (MG). The environment inside the aptamer’s electronegative binding pocket was previously harnessed to catalyze an ester ... -
Characterization of Several Small Biologically Relevant Molecules by Infrared Multiple Photon Dissociation Spectroscopy and Electronic Structure Calculations
(University of Waterloo, 2011-04-29)Infrared multiple photon dissociation (IRMPD) spectroscopy has been coupled with electronic structure calculations in order to elucidate the structures of several small biological molecules including: uracil, 5-fluorouracil, ... -
Characterization of Starch Nanoparticles by Fluorescence Techniques
(University of Waterloo, 2014-05-21)Abstract The properties of starch nanoparticles (SNPs) labeled with the fluorescent dye pyrene (Py-SNPs) were probed by using fluorescence quenching, pyrene excimer formation, and transmission electron microscopy (TEM). ... -
Characterization of the Behaviour of Solution-Responsive Polymers by Fluorescence
(University of Waterloo, 2014-04-17)Two families of amphiphilic polymers were characterized with respect to their ability to respond to changing solution conditions using steady-state and time-resolved fluorescence. Firstly, a series of 5 sequential amphiphilic ... -
Characterization of the Interactions Between DNA and Oppositely Charged Species by Fluorescence
(University of Waterloo, 2011-01-24)The characterization of the interactions between DNA and oppositely charged species were characterized by fluorescence. Quantitative and insightful information on the distribution of positively charged species around the ... -
Characterization of the Self-Assembly of Pyrene-Labelled Macromolecules in Water
(University of Waterloo, 2010-05-19)The self-assembly of several pyrene-labelled amphiphilic macromolecules in water was characterized by fluorescence. Information on their self-assembly was obtained by monitoring the level of pyrene aggregation in solution. ... -
Characterization of Various Pyrene-Labelled Macromolecules in Solution by Fluorescence
(University of Waterloo, 2010-11-04)Time-resolved fluorescence was applied to linear and branched pyrene-labelled macromolecules to study their internal dynamics. The linear macromolecules consisted of two series of pyrene-labelled poly(N-isopropylacrylamide)s ... -
Chlorine, Fluorine and Water in the Stratosphere: Chemistry, Transport and Trends based on ACE-FTS measurements
(University of Waterloo, 2006)The Atmospheric Chemistry Experiment (ACE) is a satellite mission for remote sensing of the Earth's atmosphere using the solar occultation technique. The primary instrument on this satellite is the Atmospheric Chemistry ... -
Cold Fiber Solid Phase Microextraction
(University of Waterloo, 2008-05-29)A cold fiber solid phase microextraction device was designed and constructed based on the use of a thermoelectric cooler (TEC). A three-stage thermoelectric cooler was used for cooling a copper rod coated with a ... -
Cold fiber solid phase microextraction in solid sample analysis
(University of Waterloo, 2013-05-03)The cold fiber solid phase microextraction (SPME) system was improved by minimizing the coating temperature fluctuation range, and the performance of the system was evaluated by investigating the extraction of PAHs from ... -
Combined Use of Models and Measurements for Spatial Mapping of Concentrations and Deposition of Pollutants
(University of Waterloo, 2004)When modelling pollutants in the atmosphere, it is nearly impossible to get perfect results as the chemical and mechanical processes that govern pollutant concentrations are complex. Results are dependent on the quality ...