Browsing Waterloo Institute for Nanotechnology by Subject "DNA"
Now showing items 1-20 of 62
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Accelerating peroxidase mimicking nanozymes using DNA
(Royal Society of Chemistry, 2015-09-07)DNA-capped iron oxide nanoparticles are nearly 10-fold more active as a peroxidase mimic for TMB oxidation than naked nanoparticles. To understand the mechanism, the effect of DNA length and sequence is systematically ... -
Adsorption and Desorption of DNA on Graphene Oxide Studied by Fluorescently Labeled Oligonucleotides
(American Chemical Society, 2011-03-15)Being the newest member of the carbon materials family, graphene possesses many unique physical properties resulting is a wide range of applications. Recently, it was discovered that graphene oxide can effectively adsorb ... -
Adsorption of DNA Oligonucleotides by Titanium Dioxide Nanoparticles
(American Chemical Society, 2014-01-28)Titanium dioxide (TiO2) or titania shows great promise in detoxification and drug delivery. To reach its full potential, it is important to interface TiO2 with biomolecules to harness their molecular recognition function. ... -
Adsorption of DNA onto gold nanoparticles and graphene oxide: surface science and applications
(Royal Society of Chemistry, 2012-06-28)The interaction between DNA and inorganic surfaces has attracted intense research interest, as a detailed understanding of adsorption and desorption is required for DNA microarray optimization, biosensor development, and ... -
Adsorption of Nanoceria by Phosphocholine Liposomes
(American Chemical Society, 2016-12-13)Nanoceria (CeO2 nanoparticle) possesses a number of enzyme-like activities. In particular, it scavenges reactive oxygen species based on in-vitro and in vivo antioxidation studies. An important aspect of fundamental physical ... -
Amplifying the Macromolecular Crowding Effect Using Nanoparticles
(American Chemical Society, 2012-01-11)The melting temperature (Tm) of DNA is affected not only by salt but also by the presence of high molecular weight (MW) solutes, such as polyethylene glycol (PEG), acting as a crowding agent. For short DNAs in a solution ... -
Assembly of DNA-Functionalized Gold Nanoparticles with Gaps and Overhangs in Linker DNA
(American Chemical Society, 2011-04-28)DNA-directed assembly of gold nanoparticles (AuNPs) has been extensively studied because of its important applications in analytical chemistry, materials science, and nanomedicine. In a typical system, two DNA-functionalized ... -
Assembly of DNA-Functionalized Nanoparticles in Alcoholic Solvents Reveals Opposite Thermodynamic and Kinetic Trends for DNA Hybridization
(American Chemical Society, 2010-05-12)DNA has been a key molecule in biotechnology and nanotechnology. To date, the majority of the experiments involving DNA have been performed in aqueous solutions, which may be related to the perception that DNA hybridization ... -
Biomimetic sensing based on chemically induced assembly of a signaling DNA aptamer on a fluid bilayer membrane
(Royal Society of Chemistry, 2012-04-18)The adenosine aptamer was split into two halves and linked to a fluid liposome surface; addition of adenosine resulted in aptamer assembly, which did not occur if the split aptamer was attached to silica nanoparticles, ... -
Blue emitting gold nanoclusters templated by poly-cytosine DNA at low pH and poly-adenine DNA at neutral pH
(Royal Society of Chemistry, 2012-07-11)Blue fluorescent gold nanoclusters were prepared in the presence of poly-cytosine DNAs at low pH and poly-adenine at neutral pH using citrate as the reducing agent; various buffer conditions affecting the synthesis have ... -
Boosting the oxidase mimicking activity of nanoceria by fluoride capping: rivaling protein enzymes and ultrasensitive F- detection
(Royal Society of Chemistry, 2016-07-28)Nanomaterial-based enzyme mimics (nanozymes) are currently a new forefront of chemical research. However, the application of nanozymes is limited by their low catalytic activity and low turnover numbers. Cerium dioxide ... -
Cation-Size-Dependent DNA Adsorption Kinetics and Packing Density on Gold Nanoparticles: An Opposite Trend
(American Chemical Society, 2014-11-11)The property of DNA is strongly influenced by counterions. Packing a dense layer of DNA onto a gold nanoparticle (AuNP) generates an interesting colloidal system with many novel physical properties such as a sharp melting ... -
Citrate inhibition of cisplatin reaction with DNA studied using fluorescently labeled oligonucleotides: implication for selectivity towards guanine
(Royal Society of Chemistry, 2013-10-21)The reaction between cisplatin and DNA is conveniently studied using fluorescently labeled oligonucleotides and gel electrophoresis; as an example of application, the inhibition of this reaction by citrate is demonstrated, ... -
A Comprehensive Screen of Metal Oxide Nanoparticles for DNA Adsorption, Fluorescence Quenching, and Anion Discrimination
(American Chemical Society, 2015-10-22)Although DNA has been quite successful in metal cation detection, anion detectioin remains challenging because of the charge repulsion. Metal oxides represent a very important class of materials, and different oxides might ... -
Correlation of photobleaching, oxidation and metal induced fluorescence quenching of DNA-templated silver nanoclusters
(Royal Society of Chemistry, 2013-04-07)Few-atom noble metal nanoclusters have attracted a lot of interest due to their potential applications in biosensor development, imaging and catalysis. DNA-templated silver nanoclusters (AgNCs) are of particular interest ... -
Degradable starch nanoparticle assisted ethanol precipitation of DNA
(Elsevier, 2014-04-18)Precipitation of DNA from a large volume of aqueous solution is an important step in many molecular biology and analytical chemistry experiments. Currently, this is mainly achieved by ethanol precipitation, where a long-term ... -
Dissecting the effect of anions on Hg2+ detection using a FRET based DNA probe
(Royal Society of Chemistry, 2012-08-07)Many biosensors have been developed to detect Hg2+ using thymine-rich DNA. While sensor response to various cations is often studied to demonstrate selectivity, the effect of anions has been largely overlooked. Anions may ... -
Dissociation and Degradation of Thiol-Modified DNA on Gold Nanoparticles in Aqueous and Organic Solvents
(American Chemical Society, 2011-05-17)Gold nanoparticles functionalized with thiol-modified DNA have been widely used in making various nanostructures, colorimetric biosensors, and drug delivery vehicles. Over the past 15 years, significant progress has been ... -
DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection
(Royal Society of Chemistry, 2014-05-01)Iron oxide nanoparticles adsorb fluorescently labeled DNA oligonucleotides via the backbone phosphate and quench fluorescence. Arsenate displaces adsorbed DNA to increase fluorescence, allowing detection of arsenate down ... -
DNA duplex stabilization in crowded polyanion solutions
(Royal Society of Chemistry, 2013-02-14)The melting temperature of duplex DNA is much higher in polyanions than in non-ionic polymers with similar ionic strength, suggesting an additional electrostatic contribution on top of the excluded volume effect.