Degradable starch nanoparticle assisted ethanol precipitation of DNA
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Date
2014-04-18
Authors
Ip, Alexander C-F.
Tsai, Tsung Hao
Khimji, Imran
Huang, Po-Jung Jimmy
Liu, Juewen
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Precipitation of DNA from a large volume of aqueous solution is an important step in many molecular biology and analytical chemistry experiments. Currently, this is mainly achieved by ethanol precipitation, where a long-term incubation (usually overnight) at low temperature of −20 to −80 °C with high salt concentration is required. This method also requires a large quantity of DNA to form a visible pellet and was tested mainly for double-stranded DNA. To improve DNA precipitation, co-precipitating polymers such as linear polyacrylamide has been used. In this work, we report that starch nanoparticles (SNPs) can achieve convenient DNA precipitation at room temperature with a low salt concentration and short incubation time. This method requires as low as 0.01–0.1% SNPs and can precipitate both single- and double-stranded DNA of various lengths. The effect of salt concentration, pH and the crosslinking density of SNPs has been systematically studied. Compared to other types of precipitating agents, SNPs are highly biocompatible and can be degraded by a common enzyme (amylase). This work suggests a novel application of a bio-based material that is prepared in mass production.
Description
The final publication is available at Elsevier via http://dx.doi.org/10.1016/j.carbpol.2014.04.007 © 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords
Starch, DNA, Ethanol, Fluorescence, Biosensors