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Accelerating peroxidase mimicking nanozymes using DNA

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Liu_Juewen(16).pdf (857.33 KB)

Date

2015-09-07

Authors

Liu, Biwu
Liu, Juewen

Journal Title

Journal ISSN

Volume Title

Publisher

Royal Society of Chemistry

Abstract

DNA-capped iron oxide nanoparticles are nearly 10-fold more active as a peroxidase mimic for TMB oxidation than naked nanoparticles. To understand the mechanism, the effect of DNA length and sequence is systematically studied, and other types of polymers are also compared. This rate enhancement is more obvious with longer DNA and, in particular, poly-cytosine. Among the various polymer coatings tested, DNA offers the highest rate enhancement. A similar acceleration is also observed for nanoceria. On the other hand, when the positively charged TMB substrate is replaced by the negatively charged ABTS, DNA inhibits oxidation. Therefore, the negatively charged phosphate backbone and bases of DNA can increase TMB binding by the iron oxide nanoparticles, thus facilitating the oxidation reaction in the presence of hydrogen peroxide.

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Keywords

peroxidase, nanozymes, DNA

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Citation

URI

http://dx.doi.org/10.1039/C5NR04176G
 http://hdl.handle.net/10012/11350

Collections

Waterloo Research
Chemistry
Waterloo Institute for Nanotechnology