Browsing University of Waterloo by Title
Now showing items 8825-8844 of 18769
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In Vitro Selection in Serum: RNA-Cleaving DNAzymes for Measuring Ca2+ and Mg2+
(American Chemical Society, 2016-05-27)RNA-cleaving DNAzymes have been attempted as in vivo analytical probes and gene silencing reagents over the past two decades. Despite progress already achieved, concerns still exist regarding the activity of DNAzymes in ... -
In Vitro Selection of a DNAzyme Cooperatively Binding Two Lanthanide Ions for RNA Cleavage
(American Chemical Society, 2016-05-03)Trivalent lanthanide ions (Ln(3+)) were recently employed to select RNA-cleaving DNAzymes, and three new DNAzymes have been reported so far. In this work, dysprosium (Dy3+) was used with a library containing 50 random ... -
In Vitro Selection of a New Lanthanide-Dependent DNAzyme for Ratiometric Sensing Lanthanides
(American Chemical Society, 2014-10-07)Developing biosensors for lanthanides is an important but challenging analytical task. To address this problem, in vitro selection of RNA-cleaving DNAzymes was carried out using a library containing a region of 35 random ... -
In Vitro Selection of Chromium-Dependent DNAzymes for Sensing Chromium(III) and Chromium(VI)
(Wiley, 2016-07-04)Chromium is a very important analyte for environmental monitoring, and developing biosensors for chromium is a long-standing analytical challenge. In this work, in vitro selection of RNA-cleaving DNAzymes was carried out ... -
In Vitro Selection of New DNAzymes as Metal Biosensors
(University of Waterloo, 2015-06-26)In vitro selection of metal-specific DNAzymes has been shown to be a powerful method to obtain biosensors for metal ion detection. With 14 independent in vitro selection experiments using each of the trivalent lanthanide ... -
In Vitro Selection, Characterization, and Application of a Sodium-Dependent DNAzyme and a Metal-Independent Ribozyme
(University of Waterloo, 2021-09-20)Ribozymes and DNAzymes are collectively called catalytic nucleic acids. Compared to protein enzymes, catalytic nucleic acids are programmable in structure, easy to synthesize or modify, and more chemically stable especially ... -
In Vitro Studies of a New Radiosensitizer for Radiotherapy of Breast Cancer
(University of Waterloo, 2015-08-27)Breast cancer is the most commonly diagnosed cancer among women and is the leading cause of cancer death in both developing and developed areas. Triple-negative breast cancer, one of the subtypes of breast cancer, is ... -
In vitro Studies of Improvement in Treatment Efficiency of Photodynamic Therapy of Cancers through Near-Infrared/Bioluminescent Activation
(University of Waterloo, 2014-05-22)Cancer is a leading cause of death that affects millions of people across the globe each year. Photodynamic therapy (PDT) is a relatively new treatment approach for cancer in which anticancer drugs are activated by light ... -
In vitro studies of NOS inhibitors using purified human NOS isozymes
(University of Waterloo, 2017-12-18)Endogenous nitric oxide (NO) is derived from L-arginine via a chemical reaction catalyzed by nitric oxide synthases (NOS). Three NOS isozymes are found in humans: neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible ... -
In vitro test of a potential hypoxic radiosensitizer
(University of Waterloo, 2016-09-28)Oxygen has long been known as a potent radiosensitizer that can enhance the cell killing effects in aerobic cells, while pruned vasculatures and lack of oxygen in the microenvironment of tumor can cause resistance to ... -
In vitro ultraviolet–induced damage in human corneal, lens, and retinal pigment epithelial cells
(Molecular Vision, 2011-01-21)Purpose: The purpose was to develop suitable in vitro methods to detect ocular epithelial cell damage when exposed to UV radiation, in an effort to evaluate UV-absorbing ophthalmic biomaterials.Methods: Human corneal ... -
In Vivo Calibration Methods of SPME and Application to Pharmacokinetic Studies
(University of Waterloo, 2009-05-21)Solid phase microextraction (SPME) has gained much popularity for in vivo applications recently. Thus far, there are two types of pre-equilibrium kinetic calibration that have been applied to in vivo SPME: on-fibre ... -
In Vivo Detection of Trace Organic Contaminants in Fish Using Solid Phase Microextraction
(University of Waterloo, 2010-10-21)The feasibility of using solid phase micro-extraction (SPME) as an in vivo sampling tool for analysis of trace environmental contaminants in fish exposed to municipal wastewater effluents (MWWEs) was validated using ... -
IN VIVO EPIGENETIC STUDY OF HISTONE ACETYLATION ASSOCIATED WITH OBESITY
(University of Waterloo, 2007-10-02)Post translational modifications in histone proteins are transmissible changes that are not coded for in the DNA sequence itself but have a significant affect in the control of gene expression. Eukaryotic transcription is ... -
In Vivo Imaging of Corneal Conditions using Optical Coherence Tomography
(University of Waterloo, 2006)Purposes: To use optical coherence tomography (OCT) to image and quantify the effect of various corneal conditions, in terms of corneal, stromal and epithelial thickness, and light backscatter. To assess the changes ... -
In vivo Imaging of Light Induced Intrinsic Optical Signals in the Chicken Retina with a Combined Ultra-High Resolution Optical Coherence Tomography and Electroretinography System
(University of Waterloo, 2013-01-15)The main objective of this thesis is to investigate the intrinsic optical signals (IOSs) with an ultra-high resolution optical coherence tomography system (UHROCT). In order to study the retinal IOSs evoked by visible ... -
In vivo microsampling to capture the elusive exposome
(Nature Publishing Group, 2017-03-07)Loss and/or degradation of small molecules during sampling, sample transportation and storage can adversely impact biological interpretation of metabolomics data. In this study, we performed in vivo sampling using solid-phase ... -
In vivo Solid Phase Microextraction for Brain Tissue Analysis
(University of Waterloo, 2014-03-31)New solid phase microextraction (SPME) method was developed for brain tissue bioanalysis on a liquid chromatography mass spectrometry platform. To achieve set objectives, in vivo SPME desorption process was optimized for ... -
An in vivo study of compulsions
(Elsevier, 2021-07)Leading models of obsessive-compulsive disorder (OCD) assume that compulsions are enacted to neutralize distress over obsessions. However, lab-based research suggests that once a compulsion starts factors other than distress ... -
“In Works of Hands or of the Wits of Men”: The Elegies of Wim Wenders, Laurie Anderson and Alexander Sokurov
(University of Waterloo, 2019-04-22)This dissertation explores the concept of loss and the possibility of consolation in Wim Wenders’s The Salt of the Earth, Laurie Anderson’s Heart of a Dog and Alexander Sokurov’s Oriental Elegy through a method that ...