Browsing Theses by Subject "apoptosis"
Now showing items 1-14 of 14
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Analysis of Programmed Cell Death in the Amnioserosa, an Extra-embryonic Epithelium in Drosophila melanogaster
(University of Waterloo, 2008-01-24)The amnioserosa (AS) is an epithelium that plays major roles in two crucial morphogenetic processes during Drosophila embryogenesis: Germ Band Retraction (GBR) and Dorsal Closure (DC). The AS is extraembryonic and as such, ... -
Apoptotic signalling and structural alterations in autophagy-deficient murine skeletal muscle
(University of Waterloo, 2014-10-15)Autophagy is a catabolic process by which the cell targets and degrades cytoplasmic materials, such as proteins and organelles. Autophagy is required for the control of muscle mass under catabolic conditions, but is also ... -
Characterization of Eukaryotic Translation Initiation Factor 5A isoforms (eIF-5A1 & eIF-5A2) using human cell lines as a model system
(University of Waterloo, 2006)Eukaryotic translation initiation factor 5A (eIF-5A) is the only known cellular protein that contains the post-translationally derived amino acid, hypusine. Initially, eIF-5A was named as a translation initiation factor ... -
Characterization of the pro-apoptotic function of eIF5A in human cancer cell lines
(University of Waterloo, 2007-09-18)Eukaryotic translation initiation factor 5A (eIF5A) is the only known protein containing the unique amino acid, hypusine. eIF5A is present in all eukaryotic cells and is highly conserved, but its function is not well ... -
Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products
(University of Waterloo, 2009-04-27)The utilization of in vitro tests with a tiered testing strategy for detection of mild ocular irritants can reduce the use of animals for testing, provide mechanistic data on toxic effects, and reduce the uncertainty ... -
The Effect of Mitochondrial Biogenesis on Apoptotic Susceptibility in L6 Myoblasts
(University of Waterloo, 2010-09-27)Mitochondria play an essential role in cell metabolism as well as apoptotic signaling. Chronic endurance exercise has been shown to increase mitochondrial content in skeletal muscle. Interestingly, endurance exercise has ... -
Examining the role of autophagy in modulating apoptotic signaling and skeletal muscle recovery after cardiotoxin injury in tamoxifen-inducible, skeletal muscle-specific Atg7 knockdown mice
(University of Waterloo, 2015-10-06)Skeletal muscle is a unique tissue composed of multinucleated, post-mitotic fibers. Despite its post-mitotic nature, skeletal muscle has an astounding ability to regenerate after injury largely thanks to muscle-specific ... -
Exploring the Cytoskeleton and Apoptosis Mechanisms in Fuchs Endothelial Corneal Dystrophy Using In Vitro Biomimetic Models and Immortalized FECD Cells
(University of Waterloo, 2023-12-14)Fuchs endothelial corneal dystrophy (FECD) is a corneal endothelium (CE) and Descemet’s membrane (DM) dysfunction symptom, which is the most common corneal endothelial disease. Despite the long history of clinical assessments ... -
In Vitro Modelling of Fuchs Endothelial Dystrophy for Corneal Endothelial Cell Apoptosis and Characterization of Human Descemets Membrane
(University of Waterloo, 2024-04-18)The corneal endothelium is a terminally differentiated tissue that is typically considered non-proliferative in vivo. Corneal endothelial dystrophies, including Fuchs' endothelial dystrophy (FED), are a significant cause ... -
An Obese Genotype Affects the Sphingolipid Signaling Pathway
(University of Waterloo, 2008-01-24)Sphingolipids are important signaling molecules regulating cell growth, cell death and differentiation, thus making them important molecules in determining the fate of a cell and in the pathogenesis of chronic illnesses. ... -
Proteomics Analysis of Protein-Producing Chinese Hamster Ovary Cells during Apoptosis in Prolonged Cultivation
(University of Waterloo, 2010-05-21)Among the factors important for maintaining productivity of recombinant proteins in mammalian cells, culture lifetime, cell density and cell viability are three simple but essential aspects that can greatly influence the ... -
The role of glutathione depletion in skeletal muscle apoptotic signalling in young and old rats
(University of Waterloo, 2010-09-27)There is substantial evidence that oxidative stress causes negative outcomes in many cell and tissue types. This is especially true of skeletal muscle, as it is continually subjected to various sources of reactive oxygen ... -
A study of innate antiviral mechanisms using fish cell lines
(University of Waterloo, 2006)Understanding basic antiviral mechanisms in vertebrates is essential for developing methods to enhance antiviral responses and promote human and animal health. In fish these antiviral mechanisms are poorly understood, ... -
Using cell lines to study factors affecting transmission of fish viruses
(University of Waterloo, 2014-01-02)Factors that can influence the transmission of aquatic viruses in fish production facilities and natural environment are the immune defense of host species, the ability of viruses to infect host cells, and the environmental ...