Browsing Theses by Subject "Fluorescence"
Now showing items 1-10 of 10
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Application of Multi-wavelength Fluorometry to Monitoring Protein Ultrafiltration
(University of Waterloo, 2009-05-21)Membrane filtration of protein solutions is influenced by a wide range of processing and physicochemical conditions. Monitoring and optimizing membrane filtration may have advantages for achieving, in a cost effective ... -
Aspects of Polymer Chain Dynamics in Solution Studied by Fluorescence
(University of Waterloo, 2008-04-14)Several pyrene-labeled polymers and polypeptides were synthesized and their chain dynamics were characterized using steady-state and time-resolved fluorescence techniques. Firstly, four series of pyrene-labeled polystyrenes ... -
Calibrating the Long-Range Backbone Dynamics of Polymers in Solution Using Two Different Models
(University of Waterloo, 2022-09-29)The fluorescence decays of a series of 41 pyrene-labeled poly(oligo(ethylene glycol)methyl ether methacrylate)s (Py-PEGnMA where n = 0, 1, 2, 3, 4, 5, 9, 16, 19) were prepared by free-radical polymerization of 1-pyrenebutyl ... -
Calmodulin Binding and Activation of Mammalian Nitric Oxide Synthases
(University of Waterloo, 2008-05-12)Calmodulin (CaM) is a ubiquitous cytosolic Ca2+-binding protein involved in the binding and regulation of more than three-hundred intracellular target proteins. CaM consists of two globular domains joined by a central ... -
Characterization of Starch Nanoparticles by Fluorescence Techniques
(University of Waterloo, 2014-05-21)Abstract The properties of starch nanoparticles (SNPs) labeled with the fluorescent dye pyrene (Py-SNPs) were probed by using fluorescence quenching, pyrene excimer formation, and transmission electron microscopy (TEM). ... -
Long Range Polymer Chain Dynamics Probed with Pyrene Excimer Fluorescence
(University of Waterloo, 2016-09-27)A series of fluorescently labeled vinyl polymers bearing a C1-C18 side-chain (namely poly(methyl methacrylate), poly(butyl methacrylate), poly(octyl methacrylate), poly(lauryl methacrylate), and poly(stearyl methacrylate) ... -
Performance of Biological Filters for Drinking Water Treatment and Their Use for High Pressure Membrane Biofouling Control
(University of Waterloo, 2016-01-18)Biofiltration is a promising green drinking water treatment technology that can reduce the concentration of biodegradable organic matter (BOM) in water. Direct biofiltration or biofiltration without pretreatment (BFwp) ... -
Quantitative Characterization of Polymeric Engine Oil Additives in Solution by Fluorescene
(University of Waterloo, 2015-08-24)The solution behavior of several polymeric oil additives has been characterized by using fluorescence. First, the chemical composition of the polyisobutylene-based dispersants was determined by using the inherent fluorescence ... -
Streptococcus agalactiae CAMP factor: Functional Roles of N- and C-terminal Domains & Target Membrane Effect on Toxin Activity
(University of Waterloo, 2018-05-22)CAMP factor is a well-known pore-forming toxin secreted by Streptococcus agalactiae that forms discrete pores on susceptible membranes whether natural or artificial. This work reports haemolysis and liposome permeabilization ... -
The Structure and Internal Dynamics of Polypeptides Probed with Pyrene Excimer Fluorescence
(University of Waterloo, 2021-01-25)A combination of pyrene excimer fluorescence, fluorescence blob model (FBM), and molecular mechanics optimizations (MMOs) was applied to characterize a series of pyrene-labeled polypeptides. Analysis of the fluorescence ...