Browsing University of Waterloo by Subject "fluorescence"
Now showing items 1-20 of 30
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Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine
(American Chemical Society, 2012-04-25)With a low optical background, high loading capacity, and good biocompatibility, hydrogels are ideal materials for immobilization of biopolymers to develop optical biosensors. We recently immobilized mercury and lead binding ... -
Automatic Identification of Algae using Low-cost Multispectral Fluorescence Digital Microscopy, Hierarchical Classification & Deep Learning
(University of Waterloo, 2019-12-05)Harmful algae blooms (HABs) can produce lethal toxins and are a rising global concern. In response to this threat, many organizations are monitoring algae populations to determine if a water body might be contaminated. ... -
Characterization of PIBSI Dispersants and Their PIBSA Components by Pyrene Excimer Fluorescence
(University of Waterloo, 2020-12-18)Methods are introduced to characterize the products resulting from the coupling of a polyisobutylene terminated at one end with a succinic anhydride (PIBSA) and a polyamine to generate PIBSI products where the polyamine ... -
Characterization of Polysaccharides in Starch using Fluorescence Techniques
(University of Waterloo, 2020-09-25)Amylose and amylopectin, which are the two main constituents of starch, were characterized using the pyrene excimer fluorescence (PEF) technique. First, an amylose sample was randomly labeled with the dye pyrene to yield ... -
Characterization of the Behaviour of Solution-Responsive Polymers by Fluorescence
(University of Waterloo, 2014-04-17)Two families of amphiphilic polymers were characterized with respect to their ability to respond to changing solution conditions using steady-state and time-resolved fluorescence. Firstly, a series of 5 sequential amphiphilic ... -
Characterization of the Conformation and Internal Backbone Dynamics of a-Polyglucans by Pyrene Excimer Fluorescence
(University of Waterloo, 2021-11-23)The conformation and internal backbone dynamics (IBD) of several pyrene-labeled polysaccharides (Py-PSs), namely Py-Amylopectin, Py-Glycogen, Py-Dextran, and Py-Pullulan, were successfully examined with a combination of ... -
Characterization of the Self-Assembly of Pyrene-Labelled Macromolecules in Water
(University of Waterloo, 2010-05-19)The self-assembly of several pyrene-labelled amphiphilic macromolecules in water was characterized by fluorescence. Information on their self-assembly was obtained by monitoring the level of pyrene aggregation in solution. ... -
Characterizing Polymers with Complex Architecture using Pyrene Excimer Fluorescence
(University of Waterloo, 2021-09-29)This thesis combines two different models, namely the model free analysis (MFA) and the fluorescence blob model (FBM), that were used to study the conformation and internal dynamics of two highly branched macromolecules, ... -
Citrate inhibition of cisplatin reaction with DNA studied using fluorescently labeled oligonucleotides: implication for selectivity towards guanine
(Royal Society of Chemistry, 2013-10-21)The reaction between cisplatin and DNA is conveniently studied using fluorescently labeled oligonucleotides and gel electrophoresis; as an example of application, the inhibition of this reaction by citrate is demonstrated, ... -
Correlation of photobleaching, oxidation and metal induced fluorescence quenching of DNA-templated silver nanoclusters
(Royal Society of Chemistry, 2013-04-07)Few-atom noble metal nanoclusters have attracted a lot of interest due to their potential applications in biosensor development, imaging and catalysis. DNA-templated silver nanoclusters (AgNCs) are of particular interest ... -
Daptomycin: studies on its action mode and on bacterial resistance with model membranes
(University of Waterloo, 2016-05-16)Bacterial resistance to antibiotics is one of the most serious problems facing medicine today. Recently, reports have appeared describing bacteria that are resistant to daptomycin (dap), an important antibiotic used against ... -
DNA stabilized silver nanoclusters for ratiometric and visual detection of Hg2+ and its immobilization in hydrogels
(Elsevier, 2013-04-11)DNA oligomers are particularly interesting templates for making silver nanoclusters (AgNCs) as different emission colors can be obtained by varying the DNA sequence. Many AgNCs have been used as Hg2+ sensors since Hg2+ ... -
DNA templated fluorescent gold nanoclusters reduced by Good’s buffer: from blue emitting seeds to red and near infrared emitters
(NRC Research Press, 2015-01-22)DNA-templated fluorescent gold nanoclusters (AuNCs) have been recently prepared showing higher photostability than the silver counterpart. In this work, we examined the effect of pH, DNA length, DNA sequence, and reducing ... -
DNA-Length-Dependent Fluorescence Signaling on Graphene Oxide Surface
(Wiley, 2012-02-10)Fluorescence energy transfer to graphene oxide is studied using covalently linked DNA probes ranging from 4 to 70 base pairs. The characteristic distance and mechanism of energy transfer are reported. -
Fluorescence and Adaptation of Color Images
(University of Waterloo, 2011-04-26)Color plays a vitally important role in the world we live in. It surrounds us everywhere we go. Achromatic life, restricted to black, white and grey, is extremely dull. Color fascinates artists, for it adds enormously ... -
Fluorescently Labeled Latex Particles to Monitor Film Formation
(University of Waterloo, 2017-10-25)The influence of the length of an oligo(ethylene glycol) spacer (EGn, where n is the number of ethylene glycol units) in a 1-pyrenemethoxy-EGn methacrylate (PyEG-MA) monomer on the incorporation of the monomer into a ... -
Functional nucleic acids for detecting bacteria
(De Gruyter, 2013-01-31)Bacterial infection represents one of the leading causes of disease and death, and as such, bacterial detection is an important step in managing infectious diseases. The current protocol requires growing cell cultures, ... -
Interfacing fluorescent DNA oligonucleotides with graphene oxide and metal oxides: from adsorption to sensing
(University of Waterloo, 2022-08-18)DNA, apart from being the mode of genomic information storage, has found several uses in catalysis (DNAzymes) and target detection (aptamers). Developing novel biosensors utilizing these properties has therefore been a ... -
Light-Activated Metal-Coordinated Supramolecular Complexes with Charge-Directed Self-Assembly
(American Chemical Society, 2013-02-21)Metal-coordinated materials are attractive for many applications including catalysis, sensing, and controlled release. Adenine and its derivatives have been widely used to generate many coordination complexes, polymers, ... -
Pyrene Excimer Formation: A Tool to Study Macromolecular Conformations in Solution
(University of Waterloo, 2024-04-26)The current view for fluorescence collisional quenching (FCQ) experiments is that no quantitative information can be retrieved from macromolecules containing more than a single dye-quencher pair attached at two specific ...