Browsing Science (Faculty of) by Author "Ding, Jinsong"
Now showing items 1-15 of 15
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2-Aminopurine-modified DNA homopolymers for robust and sensitive detection of mercury and silver
Zhou, Wenhu; Ding, Jinsong; Liu, Juewen (Elsevier, 2017-01-15)Heavy metal detection is a key topic in analytical chemistry. DNA-based metal recognition has advanced significantly producing many specific metal ligands, such as thymine for Hg2+ and cytosine for Ag+. For practical ... -
Aptamer-based biosensors for biomedical diagnostics
Zhou, Wenhu; Huang, Po-Jung Jimmy; Ding, Jinsong; Liu, Juewen (Royal Society of Chemistry, 2014-06-07)Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most aptamers are obtained through a combinatorial biology technique called SELEX. Since aptamers can be isolated to bind to almost any ... -
Cr3+ Binding to DNA Backbone Phosphate and Bases: Slow Ligand Exchange Rates and Metal Hydrolysis
Zhou, Wenhu; Yu, Tianmeng; Vazin, Mahsa; Ding, Jinsong; Liu, Juewen (American Chemical Society, 2017-06-07)The interaction between chromium ions and DNA is of great interest in inorganic chemistry, toxicology, and analytical chemistry. Most previous studies focused on in situ reduction of Cr(VI), producing Cr3+ for DNA binding. ... -
DNAzyme Hybridization, Cleavage, Degradation and Sensing in Undiluted Human Blood Serum
Zhou, Wenhu; Chen, Qingyun; Huang, Po-Jung Jimmy; Ding, Jinsong; Liu, Juewen (American Chemical Society, 2015-04-07)RNA-cleaving DNAzymes provide a unique platform for developing biosensors. However, a majority of the work has been performed in clean buffer solutions, while the activity of some important DNAzymes in biological sample ... -
A DNAzyme requiring two different metal ions at two distinct sites
Zhou, Wenhu; Zhang, Yupei; Huang, Po-Jung Jimmy; Ding, Jinsong; Liu, Juewen (Oxford University Press, 2015-12-10)Most previously reported RNA-cleaving DNAzymes require only a single divalent metal ion for catalysis. We recently reported a general trivalent lanthanide-dependent DNAzyme named Ce13d. This work shows that Ce13d requires ... -
An Efficient Lanthanide-Dependent DNAzyme Cleaving 2 '-5 '-Linked RNA
Zhou, Wenhu; Ding, Jinsong; Liu, Juewen (Wiley, 2016-05-17)RNA can form two types of linkage. In addition to the predominant 3'-5' linkage, 2'-5'-linked RNA is also important in biology, medicine, and prebiotic studies. Here, in vitro selection was used to isolate a DNAzyme that ... -
Fluorescent sensors using DNA-functionalized graphene oxide
Liu, Zhenbao; Liu, Biwu; Ding, Jinsong; Liu, Juewen (Springer, 2014-07-02)In the past few years, graphene oxide (GO) has emerged as a unique platform for developing DNA-based biosensors, given the DNA adsorption and fluorescence-quenching properties of GO. Adsorbed DNA probes can be desorbed ... -
A highly specific sodium aptamer probed by 2-aminopurine for robust Na+ sensing
Zhou, Wenhu; Ding, Jinsong; Liu, Juewen (Oxford University Press, 2016-09-20)Sodium is one of the most abundant metals in the environment and in biology, playing critical ecological and physiological roles. Na+ is also the most common buffer salt for nucleic acids research, while its specific ... -
In Vitro Selection in Serum: RNA-Cleaving DNAzymes for Measuring Ca2+ and Mg2+
Zhou, Wenhu; Zhang, Yupei; Ding, Jinsong; Liu, Juewen (American Chemical Society, 2016-05-27)RNA-cleaving DNAzymes have been attempted as in vivo analytical probes and gene silencing reagents over the past two decades. Despite progress already achieved, concerns still exist regarding the activity of DNAzymes in ... -
In Vitro Selection of Chromium-Dependent DNAzymes for Sensing Chromium(III) and Chromium(VI)
Zhou, Wenhu; Vazin, Mahsa; Yu, Tianmeng; Ding, Jinsong; Liu, Juewen (Wiley, 2016-07-04)Chromium is a very important analyte for environmental monitoring, and developing biosensors for chromium is a long-standing analytical challenge. In this work, in vitro selection of RNA-cleaving DNAzymes was carried out ... -
Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement
Liu, Zhenbao; Chen, Shanshan; Liu, Biwu; Wu, Jianping; Zhou, Yanbin; He, Lingyun; Ding, Jinsong; Liu, Juewen (American Chemical Society, 2014-12-16)Fluorescent aptamer probes physisorbed on graphene oxide (GO) have recently emerged as a useful sensing platform. A signal is generated by analyte-induced probe desorption. To address nonspecific probe displacement and the ... -
A New Na+-Dependent RNA-Cleaving DNAzyme with over 1000-fold Rate Acceleration by Ethanol
Zhou, Wenhu; Saran, Runjhun; Chen, Qingyun; Ding, Jinsong; Liu, Juewen (Wiley, 2016-01-15)Enzymes working in organic solvents are important for analytical chemistry, catalysis, and mechanistic studies. Although a few protein enzymes are highly active in organic solvents, little is known regarding nucleic ... -
A platinum shell for ultraslow ligand exchange: unmodified DNA adsorbing more stably on platinum than thiol and dithiol on gold
Zhou, Wenhu; Ding, Jinsong; Liu, Juewen (Royal Society of Chemistry, 2015-08-04)Due to the ultraslow ligand exchange rate on Pt, non-thiolated DNA is adsorbed on platinum nanoparticles (PtNPs) more stably than thiolated and even dithiolated DNA on AuNPs. Adsorption kinetics, capacity and stability are ... -
A Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescence
Zhou, Wenhu; Ding, Jinsong; Liu, Juewen (Wiley, 2016-08-17)A previous study of two RNA-cleaving DNAzymes, NaA43 and Ce13d, revealed the possibility of a common Na+ aptamer motif. Because Na+ binding to DNA is a fundamental biochemical problem, the interaction between Ce13d and Na+ ... -
Tandem Phosphorothioate Modifications for DNA Adsorption Strength and Polarity Control on Gold Nanoparticles
Zhou, Wenhu; Wang, Feng; Ding, Jinsong; Liu, Juewen (American Chemical Society, 2014-09-10)Unmodified DNA was recently used to functionalize gold nanoparticles via DNA base adsorption. Compared to thiolated DNA, however, the application of unmodified DNA is limited by the lack of sequence generality, adsorption ...