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Fluorescent sensors using DNA-functionalized graphene oxide

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Date

2014-07-02

Authors

Liu, Zhenbao
Liu, Biwu
Ding, Jinsong
Liu, Juewen

Advisor

Journal Title

Journal ISSN

Volume Title

Publisher

Springer

Abstract

In the past few years, graphene oxide (GO) has emerged as a unique platform for developing DNA-based biosensors, given the DNA adsorption and fluorescence-quenching properties of GO. Adsorbed DNA probes can be desorbed from the GO surface in the presence of target analytes, producing a fluorescence signal. In addition to this initial design, many other strategies have been reported, including the use of aptamers, molecular beacons, and DNAzymes as probes, label-free detection, utilization of the intrinsic fluorescence of GO, and the application of covalently linked DNA probes. The potential applications of DNA-functionalized GO range from environmental monitoring and cell imaging to biomedical diagnosis. In this review, we first summarize the fundamental surface interactions between DNA and GO and the related fluorescence-quenching mechanism. Following that, the various sensor design strategies are critically compared. Problems that must be overcome before this technology can reach its full potential are described, and a few future directions are also discussed.

Description

The final publication is available at Springer via http://dx.doi.org/10.1007/s00216-014-7888-3

Keywords

Graphene, DNA, Biosensors, Adsorption, Fluorescence

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