An Efficient Lanthanide-Dependent DNAzyme Cleaving 2 '-5 '-Linked RNA

Loading...
Thumbnail Image

Date

2016-05-17

Authors

Zhou, Wenhu
Ding, Jinsong
Liu, Juewen

Advisor

Journal Title

Journal ISSN

Volume Title

Publisher

Wiley

Abstract

RNA can form two types of linkage. In addition to the predominant 3'-5' linkage, 2'-5'-linked RNA is also important in biology, medicine, and prebiotic studies. Here, in vitro selection was used to isolate a DNAzyme that specifically cleaves 2'-5' RNA by using Ce3+ as the metal cofactor, but leaves the 3'-5' counterpart intact. This Ce5 DNAzyme requires trivalent light lanthanide ions and shows a rate of 0.16 min(-1) in the presence of 10 mm Ce3+; the activity decreases with heavier lanthanide ions. This is the fastest DNAzyme reported for this reaction, and it might enable applications in chemical biology. As a proof-of-concept, using this DNAzyme, the reactions between phosphorothioate-modified RNA and strongly thiophilic metals (Hg2+ and Tl3+) were studied as a function of pH.

Description

This is the peer reviewed version of the following article: Zhou, W., Ding, J., & Liu, J. (2016). An Efficient Lanthanide-Dependent DNAzyme Cleaving 2 ’-5 ’-Linked RNA. Chembiochem, 17(10), 890–894, which has been published in final form at https://doi.org/10.1002/cbic.201500690. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

Keywords

DNA Enzymes, Metal, Deoxyribozymes, Selectivity, Catalysis, Cofactors, Ligase, Beacon, Desulfurization, Linkages

LC Keywords

Citation