Browsing Theses by Subject "mass spectrometry"
Now showing items 1-16 of 16
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Application of Solid Phase Microextraction for Lipid Analysis and Lipidomics
(University of Waterloo, 2016-01-29)Although the latest advancements in lipid analysis technology provide an excellent opportunity for the comprehensive study of lipidome, fulfilling the analytical requirements at the desired quality is still a major ... -
Automated High-throughput Analysis of Multi-class Multi-residue Pharmaceutical Drugs in Animal Tissue Using Solid Phase Microextraction
(University of Waterloo, 2020-05-06)The use of veterinary pharmaceuticals in today´s intensive farming of food-producing animals is essential for the humane treatment of animals and as well as for the prevention of outbreaks of infectious diseases. Improper ... -
Characterization of the Human Plasma Macrolipidome using Standard Reference Material 1950 - Metabolites in frozen human plasma
(University of Waterloo, 2022-01-26)Standard Reference Material (SRM) 1950 Metabolites in frozen human plasma is a pooled plasma sample created by the National Institute of Standards and Technology (NIST) to be representative of plasma from the population ... -
Deep Learning Methods for Novel Peptide Discovery and Function Prediction
(University of Waterloo, 2024-04-26)This thesis explores deep learning methods for protein identification and property prediction, encompassing two primary areas: mass spectrometry-based protein sequence identification and protein property prediction. We ... -
Development and optimization of coated blade spray - mass spectrometry for bioanalytical and clinical applications
(University of Waterloo, 2018-08-23)Bioanalysis is a sub-discipline of analytical chemistry that focuses on the identification and quantification of small and large molecules (e.g. metabolites and proteins) present in biological sample matrices (e.g. blood, ... -
Fatty Acyl-Specific Macrolipidomics and Microlipidomics for Nutritional Research
(University of Waterloo, 2019-08-13)The field of lipidomics can further our understanding of the biochemical processes of human health and disease. Generally, lipidomics methods utilize high-performance liquid chromatography (HPLC) for lipid separation, ... -
The Investigation of Isomers using Differential Mobility Spectrometry and Ultraviolet Photodissociation Spectroscopy
(University of Waterloo, 2022-10-26)The analysis of complex mixtures in mass spectrometry (MS) is often complicated by the presence of isomers exhibiting the same mass-to-charge ratios. Tandem MS can simplify isomer analysis and enable ion characterization ... -
Laser Desorption Solid Phase Microextraction
(University of Waterloo, 2006)The use of laser desorption as a sample introduction method for solid phase microextraction (SPME) has been investigated in this research project. Three different types of analytical instruments, mass spectrometry (MS), ... -
A Machine-Learning-Based Algorithm for Peptide Feature Detection from Protein Mass Spectrometry Data
(University of Waterloo, 2021-05-13)Liquid chromatography with tandem mass spectrometry (LC-MS/MS) has been widely used in proteomics. Two types of data, MS and MS/MS data, are produced in an LC- MS/MS experiment. The MS data contains signal peaks corresponding ... -
Peptide Sequencing with Deep Learning
(University of Waterloo, 2020-09-30)In shotgun proteomics, de novo peptide sequencing from tandem mass spectrometry data is the key technology for finding new peptide or protein sequences. It has successful applications in assembling monoclonal antibody ... -
Quantification and Profiling of Hepatic Retinoids in Freshwater Fishes by Liquid Chromatography – Tandem Mass Spectrometry
(University of Waterloo, 2018-03-26)Retinoids (vitamin A1) are a class of compounds essential for a number of physiological processes including vision and immune system function. They are stored as biologically inactive retinyl esters which are mobilized ... -
Solid phase microextraction: a versatile technique for lipid analysis
(University of Waterloo, 2023-04-26)Extensive multidisciplinary research and improvements to instrumentation and workflows are continuously enabling new insights into the role of lipids in health and disease. As with other metabolites, the accurate analysis ... -
Solid-phase microextraction as sample preparation method for metabolomics
(University of Waterloo, 2010-04-28)The main objective of the emerging field of metabolomics is the analysis of all small molecule metabolites present in a particular living system in order to provide better understanding of dynamic processes occurring in ... -
Spectroscopic interrogation of differential mobility spectrometry selected, isolated, gas-phase molecular clusters
(University of Waterloo, 2020-09-25)One of the most elusive challenges facing mass spectrometry-based methods is the study of isomers. Oftentimes, isomers produce identical fragmentation spectra that make structural elucidation and assignment from MS data ... -
Towards Understanding Differential Ion Mobility and its Applications for Analytical and Medicinal Chemistry
(University of Waterloo, 2023-08-30)This PhD thesis, titled “Towards Understanding Differential Ion Mobility Spectrometry and its Applications in Analytical and Medicinal Chemistry,” encompasses a broad effort to understand the principles that underpin ... -
Using Chemical Crosslinking and Mass Spectrometry for Protein Model Validation and Fold Recognition
(University of Waterloo, 2006)The 3D structures of proteins may provide important clues to their functions and roles in complex biological pathways. Traditional methods such as X-ray crystallography and NMR are not feasible for all proteins, while ...