Browsing Science (Faculty of) by Subject "DNAzymes"
Now showing items 1-9 of 9
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Biochemical Characterization of a Lanthanide-Dependent DNAzyme with Normal and Phosphorothioate-Modified Substrates
(American Chemical Society, 2015-10-06)A trivalent lanthanide (Ln3+)-dependent RNA-cleaving DNAzyme, Ce13d, was recently isolated via in vitro selection. Ce13d is active in the presence of all Ln3+ ions. Via introduction of a single phosphorothioate (PS) ... -
Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium
(American Chemical Society, 2015-10-20)Thallium (Tl) is a highly toxic heavy metal situated between mercury and lead in the periodic table. While its neighbors have been thoroughly studied for DNA-based sensing, little is known about thallium detection. In this ... -
The group trend of lanthanides binding to DNA and DNAzymes with a complex but symmetric pattern
(Royal Society of Chemistry, 2014-07-18)Using sensitized Tb3+ luminescence spectroscopy as a tool, binding of 14 lanthanides to a lanthanide-dependent DNAzyme is studied, where the binding affinity is symmetric across the series and the tightest binding occurs ... -
In Vitro Selection of a New Lanthanide-Dependent DNAzyme for Ratiometric Sensing Lanthanides
(American Chemical Society, 2014-10-07)Developing biosensors for lanthanides is an important but challenging analytical task. To address this problem, in vitro selection of RNA-cleaving DNAzymes was carried out using a library containing a region of 35 random ... -
In Vitro Selection, Characterization, and Application of a Sodium-Dependent DNAzyme and a Metal-Independent Ribozyme
(University of Waterloo, 2021-09-20)Ribozymes and DNAzymes are collectively called catalytic nucleic acids. Compared to protein enzymes, catalytic nucleic acids are programmable in structure, easy to synthesize or modify, and more chemically stable especially ... -
Searching for a DNAzyme version of the leadzyme
(Springer, 2015-10-12)The leadzyme refers to a small ribozyme that cleaves a RNA substrate in the presence of Pb2+. In an optimized form, the enzyme strand contains only two unpaired nucleotides. Most RNA-cleaving DNAzymes are much longer. Two ... -
Sensing Parts-per-Trillion Cd2+, Hg2+ and Pb2+ Collectively and Individually Using Phosphorothioate DNAzymes
(American Chemical Society, 2014-05-22)Cadmium, mercury, and lead are collectively banned by many countries and regions in electronic devices due to their extremely high toxicity. To date, no sensing method can detect them as a group and also individually with ... -
Tandem DNAzymes for mRNA cleavage: choice of enzyme, metal ions and the antisense effect
(Elsevier, 2015-04-01)The concept of DNAzyme-based gene silencing via mRNA cleavage was proposed over twenty years ago. A number of studies regarding intracellular gene silencing have been reported as well. However, questions have been raised ... -
Two Pb2+-specific DNAzymes with opposite trends in split-site-dependent activity
(Royal Society of Chemistry, 2014-05-04)By splitting the catalytic core of DNAzymes into two halves, two Pb2+-specific DNAzymes retain partial activity, while they show opposite trends of activity as a function of the split site, revealing important nucleotides ...