Browsing Science (Faculty of) by Author "Liu, Juewen"
Now showing items 41-60 of 124
-
DNA-Functionalized Gold Nanoparticles in Macromolecularly Crowded Polymer Solutions
Zhang, Xu; Liu, Juewen; Shin, Jeehae (American Chemical Society, 2012-11-15)DNA-functionalized gold nanoparticles (AuNPs) are one of the most commonly used reagents in nanobiotechnology. They are important not only for practical applications in analytical chemistry and drug delivery, but also for ... -
DNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detection
Baeissa, Aifan; Dave, Neeshma; Smith, Brendan D.; Liu, Juewen (American Chemical Society, 2010-12-22)Highly sensitive and selective DNA detection plays a central role in many fields of research, and various assay platforms have been developed. Compared to homogeneous DNA detection, surface-immobilized probes allow washing ... -
DNA-Length-Dependent Fluorescence Signaling on Graphene Oxide Surface
Huang, Po-Jung Jimmy; Liu, Juewen (Wiley, 2012-02-10)Fluorescence energy transfer to graphene oxide is studied using covalently linked DNA probes ranging from 4 to 70 base pairs. The characteristic distance and mechanism of energy transfer are reported. -
DNAzyme Hybridization, Cleavage, Degradation and Sensing in Undiluted Human Blood Serum
Zhou, Wenhu; Chen, Qingyun; Huang, Po-Jung Jimmy; Ding, Jinsong; Liu, Juewen (American Chemical Society, 2015-04-07)RNA-cleaving DNAzymes provide a unique platform for developing biosensors. However, a majority of the work has been performed in clean buffer solutions, while the activity of some important DNAzymes in biological sample ... -
A DNAzyme requiring two different metal ions at two distinct sites
Zhou, Wenhu; Zhang, Yupei; Huang, Po-Jung Jimmy; Ding, Jinsong; Liu, Juewen (Oxford University Press, 2015-12-10)Most previously reported RNA-cleaving DNAzymes require only a single divalent metal ion for catalysis. We recently reported a general trivalent lanthanide-dependent DNAzyme named Ce13d. This work shows that Ce13d requires ... -
Driving Adsorbed Gold Nanoparticle Assembly by Merging Lipid Gel/Fluid Interfaces
Wang, Feng; Curry, Dennis E.; Liu, Juewen (American Chemical Society, 2015-11-23)Surface forces between inorganic nanoparticles and lipid bilayer is of great relevance to biophysics, medicine, and nanobiotechnology. Adsorbed nanoparticles may influence the fluidity of the underlying lipids, which may ... -
Effects of Polyethylene Glycol on DNA Adsorption and Hybridization on Gold Nanoparticles and Graphene Oxide
Zhang, Xu; Huang, Po-Jung Jimmy; Servos, Mark R.; Liu, Juewen (American Chemical Society, 2012-10-09)Understanding the interface between DNA and nanomaterials is crucial for rational design and optimization of biosensors and drug delivery systems. For detection and delivery into cells, where high concentrations of cellular ... -
An Efficient Lanthanide-Dependent DNAzyme Cleaving 2 '-5 '-Linked RNA
Zhou, Wenhu; Ding, Jinsong; Liu, Juewen (Wiley, 2016-05-17)RNA can form two types of linkage. In addition to the predominant 3'-5' linkage, 2'-5'-linked RNA is also important in biology, medicine, and prebiotic studies. Here, in vitro selection was used to isolate a DNAzyme that ... -
Electrostatically Directed Visual Fluorescence Response of DNA-Functionalized Monolithic Hydrogels for Highly Sensitive Hg2+ Detection
Dave, Neeshma; Liu, Juewen; Joseph, Kevin A. (American Chemical Society, 2011-03-23)Hydrogels are cross-linked hydrophilic polymer networks with low optical background and high loading capacity for immobilization of biomolecules. Importantly, the property of hydrogel can be precisely controlled by changing ... -
Evaporation induced wrinkling of graphene oxide at the nanoparticle interface
Wang, Feng; Liu, Juewen (Royal Society of Chemistry, 2015-01-21)With the thickness of only a single atomic layer, graphene displays many interesting surface properties. A general observation is that wrinkles are formed on graphene oxide (GO) when it is dried in the presence of adsorbed ... -
Exploring the thermal stability of DNA-linked gold nanoparticles in ionic liquids and molecular solvents
Smith, Brendan D.; Liu, Juewen; Menhaj, Arsalsan Beg (Royal Society of Chemistry, 2012-11-01)While water is the most commonly used solvent for DNA, many co-solvents have been added for various applications. Ionic liquids (ILs) are molten salts at around room temperature. ILs have been tested as a green solvent for ... -
Extraction of DNA staining dyes from DNA using hydrophobic ionic liquids
Khimji, Imran; Doan, Krystina; Bruggeman, Kiara; Huang, Po-Jung Jimmy; Vajha, Puja; Liu, Juewen (Royal Society of Chemistry, 2013-05-18)The partition of oligonucleotides and DNA staining dyes into a few hydrophobic ionic liquids has been studied, where the oligonucleotides remain in the aqueous phase and all the DNA staining dyes are extracted in the ionic ... -
Fast assembly of non-thiolated DNA on gold surface at lower pH
Jiang, Hui; Materon, Elsa M.; Sotomayor, Maria Del Pilar Taboada; Liu, Juewen (Elsevier, 2013-12-01)In a typical protocol for attaching DNA to a gold electrode, thiolated DNA is incubated with the electrode at neutral pH overnight. Here we report fast adsorption of non-thiolated DNA oligomers on gold electrodes at acidic ... -
Fast Molecular Beacon Hybridization in Organic Solvents with Improved Target Specificity
Dave, Neeshma; Liu, Juewen (American Chemical Society, 2010-12-02)DNA hybridization is of tremendous importance in biology, bionanotechnology, and biophysics. Molecular beacons are engineered DNA hairpins with a fluorophore and a quencher labeled on each of the two ends. A target DNA can ... -
Fast pH-assisted functionalization of silver nanoparticles with monothiolated DNA
Zhang, Xu; Servos, Mark R.; Liu, Juewen (Royal Society of Chemistry, 2012-10-18)Attaching monothiolated DNA to silver nanoparticles has been achieved at pH 3.0 in 30 minutes and difficulties associated with DNA attachment to AgNPs at neutral pH have been revealed by studying DNA adsorption kinetics. -
Flow Cytometry-Assisted Detection of Adenosine in Serum with an Immobilized Aptamer Sensor
Huang, Po-Jung Jimmy; Liu, Juewen (American Chemical Society, 2010-05-15)Aptamers are single-stranded nucleic acids that can selectively bind to essentially any molecule of choice. Because of their high stability, low cost, ease of modification, and availability through selection, aptamers hold ... -
Fluorescent sensors using DNA-functionalized graphene oxide
Liu, Zhenbao; Liu, Biwu; Ding, Jinsong; Liu, Juewen (Springer, 2014-07-02)In the past few years, graphene oxide (GO) has emerged as a unique platform for developing DNA-based biosensors, given the DNA adsorption and fluorescence-quenching properties of GO. Adsorbed DNA probes can be desorbed ... -
Functional nucleic acids for detecting bacteria
Huang, Po-Jung Jimmy; Liu, Minchuan; Liu, Juewen (De Gruyter, 2013-01-31)Bacterial infection represents one of the leading causes of disease and death, and as such, bacterial detection is an important step in managing infectious diseases. The current protocol requires growing cell cultures, ... -
G-Quadruplex DNA for Fluorescent and Colorimetric Detection of Thallium(I)
Hoang, Michael; Huang, Po-Jung Jimmy; Liu, Juewen (American Chemical Society, 2016-02-26)Thallium is a highly toxic heavy metal, but its 495 nm FRET sensing is underexplored compared to its neighboring elements Blue in the periodic table: lead and mercury. Thallium has two " oxidation states. A DNAzyme-based ... -
Glutathione-s-transferase modified electrodes for detecting anticancer drugs
Materon, Elsa M.; Huang, Po-Jung Jimmy; Wong, Ademar; Ferreira, Antonio A. Pupim; Sotomayor, Maria Del Pilar Taboada; Liu, Juewen (Elsevier, 2014-03-07)With the fast growth of cancer research, new analytical methods are needed to measure anticancer drugs. This is usually accomplished by using sophisticated analytical instruments. Biosensors are attractive candidates for ...