Glutathione-s-transferase modified electrodes for detecting anticancer drugs
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Date
2014-03-07
Authors
Materon, Elsa M.
Huang, Po-Jung Jimmy
Wong, Ademar
Ferreira, Antonio A. Pupim
Sotomayor, Maria Del Pilar Taboada
Liu, Juewen
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
With the fast growth of cancer research, new analytical methods are needed to measure anticancer drugs. This is usually accomplished by using sophisticated analytical instruments. Biosensors are attractive candidates for measuring anticancer drugs, but currently few biosensors can achieve this goal. In particular, it is challenging to have a general method to monitor various types of anticancer drugs with different structures. In this work, a biosensor was developed to detect anticancer drugs by modifying carbon paste electrodes with glutathione-s-transferase (GST) enzymes. GST is widely studied in the metabolism of xenobiotics and is a major contributing factor in resistance to anticancer drugs. The measurement of anticancer drugs is based on competition between 1-chloro-2,4-dinitrobenzene (CDNB) and the drugs for the GST enzyme in the electrochemical potential at 0.1 V vs. Ag/AgCl by square wave voltammetry (SWV) or using a colorimetric method. The sensor shows a detection limit of 8.8 μM cisplatin and exhibits relatively long life time in daily measurements.
Description
The final publication is available at Elsevier via http://dx.doi.org/10.1016/j.bios.2014.02.070. © 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords
Glutathione-s-transferase, Carbon past electrode, Cisplatin, Glutathione