G-Quadruplex DNA for Fluorescent and Colorimetric Detection of Thallium(I)
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Date
2016-02-26
Authors
Hoang, Michael
Huang, Po-Jung Jimmy
Liu, Juewen
Journal Title
Journal ISSN
Volume Title
Publisher
American Chemical Society
Abstract
Thallium is a highly toxic heavy metal, but its 495 nm FRET sensing is underexplored compared to its neighboring elements Blue in the periodic table: lead and mercury. Thallium has two " oxidation states. A DNAzyme-based biosensor for Tl3+ was reported recently, representing the first work in this area. However, the most environmentally abundant thallium is monovalent Tl+, which is the focus of this work. Since Tl+ is similar to K+ in terms of size and charge, G-quadruplex DNAs are herein tested for Tl+ detection. First, nine dual fluorophore labeled DNA probes are screened. Among them, a DNA designated PS2.M has the largest increase in fluorescence resonance energy transfer (FRET) efficiency upon Tl+ addition. This FRET-based assay is directly used as a biosensor yielding a detection limit of 59 mu M Tl+. In comparison, K+ had a much lower response and the other tested monovalent metals do not produce a significant signal increase. In addition, a colorimetric sensor was developed based on DNA protected gold nanoparticles. When folded by Tl+, the nonlabeled PS2.M DNA cannot effectively adsorb onto gold nanoparticles. This leads to a color change from red to blue upon salt addition. The detection limit is 4.6 mu M Tl+, and Tl+ spiked in a lake water sample can also be detected. CD spectroscopy is used to further understand Tl+ binding to PS2.M. This study demonstrates that DNA can also be used for detecting Tl+, and this work gives rise to a highly effective probe for this purpose.
Description
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Sensors, © 2016 American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Hoang, M., Huang, P.-J. J., & Liu, J. (2016). G-Quadruplex DNA for Fluorescent and Colorimetric Detection of Thallium(I). Acs Sensors, 1(2), 137–143. https://doi.org/10.1021/acssensors.5b00147
Keywords
Gold Nanoparticles, Aqueous-Solution, Lead Ions, DNAzyme, Aptamer, Sensor, Complex, Binding, Probes, Beacon