Browsing Theses by Subject "Escherichia coli"
Now showing items 1-9 of 9
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Comparison of the methylerythritol phosphate pathway and the isopentenol utilization pathway for astaxanthin production in Escherichia coli
(University of Waterloo, 2020-09-01)Astaxanthin is a red carotenoid belonging to the isoprenoid family of compounds. Currently, the most common method of producing astaxanthin is through chemical synthesis and natural production using Haematococcus pluvialis. ... -
Disposable rocking bioreactors for recombinant protein production in Escherichia coli: Physical characterization and assessment of therapeutic protein expression
(University of Waterloo, 2013-09-26)Disposable technology has gained increasing acceptance in the biopharmaceutical industry over the last decade, and provides many advantages over conventional stainless steel equipment. Disposable rocking bioreactors (RBs) ... -
Effects of disulfide bond formation in production of the recombinant extracellular domain of human CD83 as a therapeutic protein
(University of Waterloo, 2010-09-28)The formation of aberrant disulfide bonds is a structural consideration for the manufacturing of the extracellular domain of human CD83 (hCD83ext), a potential therapeutic protein. In certain instances, hCD83ext protein ... -
Exploration of acetate as a feedstock for propionate production in engineered Escherichia coli
(University of Waterloo, 2017-08-29)With a relatively low reductance, acetate is considered as a poor and uncommon carbon source for microbial production and, therefore, the production strains will require major strain engineering for effective utilization ... -
High-Level Microbial Production of Propionate in Engineered Escherichia coli
(University of Waterloo, 2015-01-07)Biological platforms for propionate production have been limited to anaerobic native microbial producers, such as Propionibacterium and Clostridium. In this work, we demonstrated high-level heterologous production of ... -
Integrated metabolic engineering and bioprocessing strategies for production of succinyl-CoA-derived chemicals in Escherichia coli
(University of Waterloo, 2020-09-15)Due to various social, environmental, and technological issues associated with petro-based chemical processes, bio-based production using microbial cell factories has been recognized as a modern biotechnology for more ... -
Molecular and Genetic Strategies to Enhance Functional Expression of Recombinant Protein in Escherichia coli
(University of Waterloo, 2009-09-24)The versatile Escherichia coli facilitates protein expression with relative simplicity, high cell density on inexpensive substrates, well known genetics, variety of expression vectors, mutant strains, co-overexpression ... -
Production of 3-hydroxy acids from acetate in engineered Escherichia coli
(University of Waterloo, 2018-09-21)Acetate serves as an uncommon carbon source for metabolic engineering of value-added chemicals. As a carbon source, acetate has unique advantages since it is not derived from food sources and can be produced as a waste ... -
Simultaneous clarification and purification of recombinant penicillin G acylase using tangential flow filtration anion-exchange membrane chromatography
(University of Waterloo, 2012-04-09)Downstream purification often represents the most cost-intensive step in the manufacturing of recombinant proteins. Conventional purification processes are lengthy, technically complicated, product specific and time-consuming. ...