Browsing University of Waterloo by Author "Huang, Po-Jung Jimmy"
Now showing items 21-40 of 42
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Flow Cytometry-Assisted Detection of Adenosine in Serum with an Immobilized Aptamer Sensor
Huang, Po-Jung Jimmy; Liu, Juewen (American Chemical Society, 2010-05-15)Aptamers are single-stranded nucleic acids that can selectively bind to essentially any molecule of choice. Because of their high stability, low cost, ease of modification, and availability through selection, aptamers hold ... -
Functional nucleic acids for detecting bacteria
Huang, Po-Jung Jimmy; Liu, Minchuan; Liu, Juewen (De Gruyter, 2013-01-31)Bacterial infection represents one of the leading causes of disease and death, and as such, bacterial detection is an important step in managing infectious diseases. The current protocol requires growing cell cultures, ... -
G-Quadruplex DNA for Fluorescent and Colorimetric Detection of Thallium(I)
Hoang, Michael; Huang, Po-Jung Jimmy; Liu, Juewen (American Chemical Society, 2016-02-26)Thallium is a highly toxic heavy metal, but its 495 nm FRET sensing is underexplored compared to its neighboring elements Blue in the periodic table: lead and mercury. Thallium has two " oxidation states. A DNAzyme-based ... -
Glutathione-s-transferase modified electrodes for detecting anticancer drugs
Materon, Elsa M.; Huang, Po-Jung Jimmy; Wong, Ademar; Ferreira, Antonio A. Pupim; Sotomayor, Maria Del Pilar Taboada; Liu, Juewen (Elsevier, 2014-03-07)With the fast growth of cancer research, new analytical methods are needed to measure anticancer drugs. This is usually accomplished by using sophisticated analytical instruments. Biosensors are attractive candidates for ... -
The group trend of lanthanides binding to DNA and DNAzymes with a complex but symmetric pattern
Lin, Wei Ting David; Huang, Po-Jung Jimmy; Pautler, Rachel; Liu, Juewen (Royal Society of Chemistry, 2014-07-18)Using sensitized Tb3+ luminescence spectroscopy as a tool, binding of 14 lanthanides to a lanthanide-dependent DNAzyme is studied, where the binding affinity is symmetric across the series and the tightest binding occurs ... -
Hg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA
Huang, Po-Jung Jimmy; van Ballegooie, Courtney; Liu, Juewen (Royal Society of Chemistry, 2016-06-21)Mercury is a highly toxic heavy metal and many DNA-based biosensors have been recently developed for Hg2+ detection in water. Among them, thymine-rich DNA is the most commonly used for designing Hg2+ sensors. However, the ... -
Immobilization of DNA on Magnetic Microparticles for Mercury Enrichment and Detection with Flow Cytometry
Huang, Po-Jung Jimmy; Liu, Juewen (Wiley, 2011-04-26)Mercury detection in water has attracted a lot of research interest due to its highly toxic nature and adverse environmental impact. In particular, the recent discovery of specific binding of HgII to thymine-rich (T-rich) ... -
In Vitro Selection of a DNAzyme Cooperatively Binding Two Lanthanide Ions for RNA Cleavage
Huang, Po Jung Jimmy; Vazin, Mahsa; Liu, Juewen (American Chemical Society, 2016-05-03)Trivalent lanthanide ions (Ln(3+)) were recently employed to select RNA-cleaving DNAzymes, and three new DNAzymes have been reported so far. In this work, dysprosium (Dy3+) was used with a library containing 50 random ... -
In Vitro Selection of a New Lanthanide-Dependent DNAzyme for Ratiometric Sensing Lanthanides
Huang, Po-Jung Jimmy; Vazin, Mahsa; Liu, Juewen (American Chemical Society, 2014-10-07)Developing biosensors for lanthanides is an important but challenging analytical task. To address this problem, in vitro selection of RNA-cleaving DNAzymes was carried out using a library containing a region of 35 random ... -
In Vitro Selection of New DNAzymes as Metal Biosensors
Huang, Po-Jung Jimmy (University of Waterloo, 2015-06-26)In vitro selection of metal-specific DNAzymes has been shown to be a powerful method to obtain biosensors for metal ion detection. With 14 independent in vitro selection experiments using each of the trivalent lanthanide ... -
Liposome/Graphene Oxide Interaction Studied by Isothermal Titration Calorimetry
Huang, Po-Jung Jimmy; Wang, Feng; Liu, Juewen (American Chemical Society, 2015-03-15)The interaction between graphene oxide (GO) and lipid bilayers is important for fundamental surface science and many applications. In this work, isothermal titration calorimetry (ITC), cryo-TEM, and fluorescence spectroscopy ... -
Molecular Beacon Lighting up on Graphene Oxide
Huang, Po-Jung Jimmy; Liu, Juewen (American Chemical Society, 2012-05-01)A molecular beacon (MB) is comprised of a fluorophore and a quencher linked by a DNA hairpin. MBs have been widely used for homogeneous DNA detection. In addition to molecular quenchers, many nanomaterials such as graphene ... -
Nucleotide coordination with 14 lanthanides studied by isothermal titration calorimetry
Zhang, Zijie; Morishita, Kiyoshi; Lin, Wei Ting David; Huang, Po-Jung Jimmy; Liu, Juewen (Elsevier, 2017-06-09)With their hydrolytic, optical and magnetic properties, lanthanide ions (Ln3+) are versatile probes for nucleic acids. In addition, nucleotide-coordinated Ln3+ ions form useful nanoparticles. However, the thermodynamic ... -
Oxidation Level-Dependent Zwitterionic Liposome Adsorption and Rupture by Graphene-based Materials and Light-Induced Content Release
Ip, Alexander C-F.; Liu, Biwu; Huang, Po-Jung Jimmy; Liu, Juewen (Wiley, 2013-04-08)Liposomes may be stably adsorbed or ruptured on graphene-based materials, depending on the oxidation state of graphene. IR-induced liposome leakage is achieved, since graphene oxide does not induce liposome leakage spontaneously. -
Parts-per-Million of Polyethylene Glycol as a Non-Interfering Blocking Agent for Homogeneous Biosensor Development
Liu, Biwu; Huang, Po-Jung Jimmy; Zhang, Xu; Wang, Feng; Pautler, Rachel; Ip, Alexander C-F.; Liu, Juewen (American Chemical Society, 2013-11-05)Many homogeneous assays are complicated by the adsorption of probe molecules by the surface of reaction vessels, which are often made of polypropylene or polystyrene-based plastics. To solve this problem, many protein and ... -
Rationally Designed Nucleobase and Nucleotide Coordinated Nanoparticles for Selective DNA Adsorption and Detection
Wang, Feng; Liu, Biwu; Huang, Po-Jung Jimmy; Liu, Juewen (American Chemical Society, 2013-12-17)Nanomaterials for DNA adsorption are useful for sequence-specific DNA detection. Current materials for DNA adsorption employ electrostatic attraction, hydrophobic interaction, or π–π stacking, none of which can achieve ... -
Regenerable DNA-Functionalized Hydrogels for Ultrasensitive, Instrument-Free Mercury(II) Detection and Removal in Water
Dave, Neeshma; Chan, Michelle Y.; Huang, Po-Jung Jimmy; Smith, Brendan D.; Liu, Juewen (American Chemical Society, 2010-09-15)Mercury is a highly toxic environmental pollutant with bioaccumulative properties. Therefore, new materials are required to not only detect but also effectively remove mercury from environmental sources such as water. We ... -
Sensing Parts-per-Trillion Cd2+, Hg2+ and Pb2+ Collectively and Individually Using Phosphorothioate DNAzymes
Huang, Po-Jung Jimmy; Liu, Juewen (American Chemical Society, 2014-05-22)Cadmium, mercury, and lead are collectively banned by many countries and regions in electronic devices due to their extremely high toxicity. To date, no sensing method can detect them as a group and also individually with ... -
Synergistic pH effect for reversible shuttling aptamer-based biosensors between graphene oxide and target molecules
Huang, Po-Jung Jimmy; Kempaiah, Ravindra; Liu, Juewen (Royal Society of Chemistry, 2011-06-07)DNA aptamers are known to desorb from graphene oxide (GO) surface in the presence of target molecules. We demonstrate herein that the binding equilibrium can be shifted by simply tuning the solution pH. At lower pH, the ... -
Thioflavin T fluorescence and NMR spectroscopy 1 suggesting a non-G-quadruplex structure for a 2 sodium binding aptamer embedded in DNAzymes
Saran, Runjhun; Piccolo, Kyle A.; He, Yanping; Kang, Yongqiang; Huang, Po-Jung Jimmy; Wei, Chunying; Chen, Da; Dieckmann, Thorsten; Liu, Juewen (Canadian Science Publishing, 2021-09-16)Recently, a Na+-binding aptamer was reported to be embedded in a few RNA-cleaving DNAzymes including NaA43, Ce13d and NaH1. These DNAzymes require Na+ for activity but show no activity in the presence of K+ or other metal ...