Liposome/Graphene Oxide Interaction Studied by Isothermal Titration Calorimetry
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Date
2015-03-15
Authors
Huang, Po-Jung Jimmy
Wang, Feng
Liu, Juewen
Journal Title
Journal ISSN
Volume Title
Publisher
American Chemical Society
Abstract
The interaction between graphene oxide (GO) and lipid bilayers is important for fundamental surface science and many applications. In this work, isothermal titration calorimetry (ITC), cryo-TEM, and fluorescence spectroscopy were used to study the adsorption of three types of liposomes. Heat release was observed when GO was mixed with zwitterionic DOPC liposomes, while heat absorption occurred with cationic DOTAP liposomes. For comparison,. anionic DOPG liposomes released heat when mixed with DOTAP. DOPC was adsorbed as intact liposomes, but DOTAP ruptured and induced stacking and folding of GO sheets. This study suggests the release of more water molecules from the GO surface when mixed with DOTAP liposomes. This can be rationalized by the full rupture of the DOTAP liposomes interacting with the whole GO surface, including hydrophobic regions, while DOPC liposomes only interact with a small area on GO near the edge, which is likely to be more hydrophilic. This interesting biointerfacial observation has enhanced our fundamental understanding of lipid/GO interactions.
Description
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir, © DATE American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Huang, P.-J. J., Wang, F., & Liu, J. (2016). Liposome/Graphene Oxide Interaction Studied by Isothermal Titration Calorimetry. Langmuir, 32(10), 2458–2463. https://doi.org/10.1021/acs.langmuir.6b00006
Keywords
Reduced Graphene Oxide, Nano-Graphene, Membrane, Spectroscopy, Adsorption, Nanosheets, Bacteria, Delivery, Platform, Sensors