Browsing Waterloo Research by Subject "DNAzymes"
Now showing items 1-8 of 8
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Biochemical Characterization of a Lanthanide-Dependent DNAzyme with Normal and Phosphorothioate-Modified Substrates
(American Chemical Society, 2015-10-06)A trivalent lanthanide (Ln3+)-dependent RNA-cleaving DNAzyme, Ce13d, was recently isolated via in vitro selection. Ce13d is active in the presence of all Ln3+ ions. Via introduction of a single phosphorothioate (PS) ... -
Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium
(American Chemical Society, 2015-10-20)Thallium (Tl) is a highly toxic heavy metal situated between mercury and lead in the periodic table. While its neighbors have been thoroughly studied for DNA-based sensing, little is known about thallium detection. In this ... -
The group trend of lanthanides binding to DNA and DNAzymes with a complex but symmetric pattern
(Royal Society of Chemistry, 2014-07-18)Using sensitized Tb3+ luminescence spectroscopy as a tool, binding of 14 lanthanides to a lanthanide-dependent DNAzyme is studied, where the binding affinity is symmetric across the series and the tightest binding occurs ... -
In Vitro Selection of a New Lanthanide-Dependent DNAzyme for Ratiometric Sensing Lanthanides
(American Chemical Society, 2014-10-07)Developing biosensors for lanthanides is an important but challenging analytical task. To address this problem, in vitro selection of RNA-cleaving DNAzymes was carried out using a library containing a region of 35 random ... -
Searching for a DNAzyme version of the leadzyme
(Springer, 2015-10-12)The leadzyme refers to a small ribozyme that cleaves a RNA substrate in the presence of Pb2+. In an optimized form, the enzyme strand contains only two unpaired nucleotides. Most RNA-cleaving DNAzymes are much longer. Two ... -
Sensing Parts-per-Trillion Cd2+, Hg2+ and Pb2+ Collectively and Individually Using Phosphorothioate DNAzymes
(American Chemical Society, 2014-05-22)Cadmium, mercury, and lead are collectively banned by many countries and regions in electronic devices due to their extremely high toxicity. To date, no sensing method can detect them as a group and also individually with ... -
Tandem DNAzymes for mRNA cleavage: choice of enzyme, metal ions and the antisense effect
(Elsevier, 2015-04-01)The concept of DNAzyme-based gene silencing via mRNA cleavage was proposed over twenty years ago. A number of studies regarding intracellular gene silencing have been reported as well. However, questions have been raised ... -
Two Pb2+-specific DNAzymes with opposite trends in split-site-dependent activity
(Royal Society of Chemistry, 2014-05-04)By splitting the catalytic core of DNAzymes into two halves, two Pb2+-specific DNAzymes retain partial activity, while they show opposite trends of activity as a function of the split site, revealing important nucleotides ...