Browsing Science (Faculty of) by Subject "In-Vitro Selection"
Now showing items 1-8 of 8
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Distinction of Individual Lanthanide Ions with a DNAzyme Beacon Array
(American Chemical Society, 2016-06-24)Developing chemical probes to distinguish each lanthanide ion is a long-standing challenge. Aside from its analytical applications, solving this problem will also enhance our knowledge in metal ligand design. Using in vitro ... -
A DNAzyme requiring two different metal ions at two distinct sites
(Oxford University Press, 2015-12-10)Most previously reported RNA-cleaving DNAzymes require only a single divalent metal ion for catalysis. We recently reported a general trivalent lanthanide-dependent DNAzyme named Ce13d. This work shows that Ce13d requires ... -
A highly specific sodium aptamer probed by 2-aminopurine for robust Na+ sensing
(Oxford University Press, 2016-09-20)Sodium is one of the most abundant metals in the environment and in biology, playing critical ecological and physiological roles. Na+ is also the most common buffer salt for nucleic acids research, while its specific ... -
Molecularly Imprinted Polymers with DNA Aptamer Fragments as Macromonomers
(American Chemical Society, 2016-03-16)Molecularly imprinted polymers (MIPs) are produced in the presence of a template molecule. After removing the template, the cavity can selectively rebind the template. MIPs are attractive functional materials with a low ... -
A New Na+-Dependent RNA-Cleaving DNAzyme with over 1000-fold Rate Acceleration by Ethanol
(Wiley, 2016-01-15)Enzymes working in organic solvents are important for analytical chemistry, catalysis, and mechanistic studies. Although a few protein enzymes are highly active in organic solvents, little is known regarding nucleic ... -
A Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescence
(Wiley, 2016-08-17)A previous study of two RNA-cleaving DNAzymes, NaA43 and Ce13d, revealed the possibility of a common Na+ aptamer motif. Because Na+ binding to DNA is a fundamental biochemical problem, the interaction between Ce13d and Na+ ... -
A Silver DNAzyme
(American Chemical Society, 2016-04-05)Silver is a very common heavy metal, and its detection is of significant analytical importance. DNAzymes are DNA-based catalysts; they typically recruit divalent and trivalent metal ions for catalysis. Herein, we report a ... -
An Ultrasensitive Light-up Cu2+ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate
(American Chemical Society, 2017-01-15)Cu2+ is a very important metal ion in biology, environmental science, and industry. Developing biosensors for Cu2+ is a key topic in analytical chemistry. DNAzyme-based sensors are highly attractive:, for their excellent ...