A Silver DNAzyme
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Date
2016-04-05
Authors
Saran, Runjhun
Liu, Juewen
Journal Title
Journal ISSN
Volume Title
Publisher
American Chemical Society
Abstract
Silver is a very common heavy metal, and its detection is of significant analytical importance. DNAzymes are DNA-based catalysts; they typically recruit divalent and trivalent metal ions for catalysis. Herein, we report a silver specific RNA-cleaving DNAzyme named Ag10c obtained after six rounds of in vitro selection. Ag10c displays a catalytic rate of 0.41 min(-1) with 10 mu M Ag+ at pH 7.5 with 200 mM NaNO3, while its activity is completely inhibited with the same concentration of NaCl. Ag10c is highly specific for Ag+ among all the tested metals. A catalytic beacon biosensor is designed by labeling a fluorophore and a quencher on the DNAzyme. Fluorescence enhancement is observed in the presence of Ag+ with a detection limit of 24.9 nM Ag+. The sensor shows a similar analytical performance in Lake Huron water. This is the first monovalent transition metal dependent RNA-cleaving DNAzyme. Apart from its biosensor application, this study strengthens the idea of exploring beyond the traditional understanding of multivalent ion dependent DNAzyme catalysis.
Description
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © 2016 American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Saran, R., & Liu, J. (2016). A Silver DNAzyme. Analytical Chemistry, 88(7), 4014–4020. http://dx.doi.org/10.1021/acs.analchem.6b00327
Keywords
Lanthanide-Dependent DNAzyme, In-Vitro Selection, Cleaving DNA, Metal-Ions, Acid, Catalysis, Sensor, Nanoparticles, Sensitivity, Biosensor