Browsing Chemical Engineering by Supervisor "Chou, C. Perry"
Now showing items 1-6 of 6
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Application of hydrocarbon and perfluorocarbon oxygen vectors to enhance heterologous production of hyaluronic acid in engineered Bacillus subtilis
(University of Waterloo, 2017-12-19)In microbial cultivations for hyaluronic acid (HA) production, oxygen can be a limiting substrate due to its poor solubility in aqueous medium and the substantial increase in culture viscosity at relatively low HA titers. ... -
A comprehensive CRISPR-Cas9 toolkit for Bacillus subtilis: development for biomanufacturing applications
(University of Waterloo, 2018-04-30)Bacillus subtilis is a model Gram-positive GRAS organism sought after for its robust growth characteristics in inexpensive media, genetic tractability, and ability to secrete products into the extracellular environment. ... -
Exploration of acetate as a feedstock for propionate production in engineered Escherichia coli
(University of Waterloo, 2017-08-29)With a relatively low reductance, acetate is considered as a poor and uncommon carbon source for microbial production and, therefore, the production strains will require major strain engineering for effective utilization ... -
Integrated metabolic engineering and bioprocessing strategies for production of succinyl-CoA-derived chemicals in Escherichia coli
(University of Waterloo, 2020-09-15)Due to various social, environmental, and technological issues associated with petro-based chemical processes, bio-based production using microbial cell factories has been recognized as a modern biotechnology for more ... -
Manipulating the Sleeping Beauty Mutase Operon in Engineered Escherichia Coli for Controlled Biosynthesis of 1-Propanol and Other Value-Added Chemicals
(University of Waterloo, 2016-05-02)A great fraction of the world’s energy requirements are presently met through the unrestricted use of fossil-derived fuels. However, due to the anticipated demise of these energy sources and the environmental and socioeconomic ... -
Production of 3-hydroxy acids from acetate in engineered Escherichia coli
(University of Waterloo, 2018-09-21)Acetate serves as an uncommon carbon source for metabolic engineering of value-added chemicals. As a carbon source, acetate has unique advantages since it is not derived from food sources and can be produced as a waste ...