Browsing Chemical Engineering by Subject "Baculovirus"
Now showing items 1-4 of 4
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Characterization of Alternative Promoters to Stagger and Control Multiple Gene Expression and Protein Production in the Baculovirus-Insect Cell System
(University of Waterloo, 2014-11-05)The baculovirus-insect cell system is one of the most widely used systems for recombinant protein production. The ease of handling insect cells and their ability to produce mammalian-like post-translational modifications, ... -
Investigation of Sf-9 Cell Metabolism Before and After Baculovirus Infection Using Biovolume: a Case for the Improvement of Adeno-Associated Viral Vector Production
(University of Waterloo, 2009-08-07)Adeno-associated viral (AAV) vectors have been shown to be potential vectors for the treatment of diseases, including protocols using RNA interference (RNAi). AAV vector production in insect cells using the baculovirus ... -
Real Time PCR Protocol Development for Rapid and Low Cost Quantification of Baculovirus and for Monitoring Progression of Infection
(University of Waterloo, 2010-10-29)The work presented in this thesis aims to further the understanding and implementation of the Baculovirus Expression Vector System (BEVS) for varied uses such as protein production and viral vector production. To this end, ... -
Use and Control of Co-Expression in the Baculovirus-Insect Cell System for the Production of Multiple Proteins and Complex Biologics
(University of Waterloo, 2016-08-19)The ease of use and versatility of the Baculovirus Expression Vector System (BEVS) has made it one of the most widely used platforms for recombinant protein production. In the last ten years, the system has even gained ...