Browsing Engineering (Faculty of) by Author "Aucoin, Marc"
Now showing items 1-9 of 9
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Construction and analysis of a genetically tuneable lytic phage display system
Nicastro, Jessica; Sheldon, Katlyn; El-zarkout, Farah A.; Sokolenko, Stanislav; Aucoin, Marc; Slavcev, Roderick (Springer, 2013-09-01)The Bacteriophage lambda capsid protein gpD has been used extensively for fusion polypeptides that can be expressed from plasmids in Escherichia coli and remain soluble. In this study, a genetically controlled dual expression ... -
A correction method for systematic error in 1H-NMR time-course data validated through stochastic cell culture simulation
Sokolenko, Stanislav; Aucoin, Marc (BioMed Central, 2015-09-04)Background: The growing ubiquity of metabolomic techniques has facilitated high frequency time-course data collection for an increasing number of applications. While the concentration trends of individual metabolites can ... -
Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems
Thompson, Christine M.; Petiot Emma; Mullick Alaka; Aucoin, Marc; Henry Olivier; Kamen, Amine A. (BioMed Central, 2015-05-16)Background: Each year, influenza is responsible for hundreds of thousand cases of illness and deaths worldwide. Due to the virus' fast mutation rate, the World Health Organization (WHO) is constantly on alert to rapidly ... -
The effect of hydrogen peroxide produced during ultraviolet disinfection of CHO cell culture media
Dare, Emma V.; Gabriel, Michelle; Begum, Afroza; Sasges, Michael; Aucoin, Marc (Elsevier, 2017-10-01)Ultraviolet (UV) irradiation is being considered for protection against viral contamination in cell culture media. Hydrogen peroxide (H2O2) produced by UV irradiation has been suggested as the cause for poor cell growth ... -
Identifying model error in metabolic flux analysis - a generalized least squares approach
Sokolenko, Stanislav; Quattrociocchi, Marco; Aucoin, Marc (BioMed Central, 2016-09-13)Background: The estimation of intracellular flux through traditional metabolic flux analysis (MFA) using an overdetermined system of equations is a well established practice in metabolic engineering. Despite the continued ... -
Impact of dissolved oxygen during UV-irradiation on the chemical composition and function of CHO cell culture media
Meunier Sarah M.; Todorovic, Biljana; Dare, Emma V.; Begum, Afroza; Guillemette, Simon R.; Wenger, Andrew; Saxena, Priyanka; Campbell, J. Larry; Sasges, Michael; Aucoin, Marc (Public Library of Science, 2016-03-14)Ultraviolet (UV) irradiation is advantageous as a sterilization technique in the biopharmaceutical industry since it is capable of targeting non-enveloped viruses that are typically challenging to destroy, as well as smaller ... -
Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli
Srirangan, Kajan; Akawi, Lamees; Liu, Xuejia; Westbrook, Adam; Blondeel, Eric J. M.; Aucoin, Marc; Moo-Young, Murray; Chou, C. Perry (BioMed Central, 2013-09-28)Background: While most resources in biofuels were directed towards implementing bioethanol programs, 1-propanol has recently received attention as a promising alternative biofuel. Nevertheless, no microorganism has been ... -
Supplementing glycosylation: A review of applying nucleotide-sugar precursors to growth medium to affect therapeutic recombinant protein glycoform distributions
Blondeel, Eric J. M.; Aucoin, Marc (Elsevier, 2018-09-01)Glycosylation is a critical quality attribute (CQA) of many therapeutic proteins, particularly monoclonal antibodies (mAbs), and is a major consideration in the approval of biosimilar biologics due to its effects to ... -
Treating Cell Culture Media with UV Irradiation against Adventitious Agents: Minimal Impact on CHO Performance
Yen, Sandi; Sokolenko, Stanislav; Manocha, Bhavik; Blondeel, Eric J. M.; Aucoin, Marc; Patras, Ankit; Daynouri-Pancino, Farnaz; Sasges, Michael (Wiley, 2014-10-01)Sterility of cell culture media is an important concern in biotherapeutic processing. In large scale biotherapeutic production, a unit contamination of cell culture media can have costly effects. Ultraviolet (UV) irradiation ...