Browsing Theses by Subject "RNA"
Now showing items 1-8 of 8
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Biochemical Characterization of Fluorophore-Binding Nucleic Acids
(University of Waterloo, 2022-09-27)Aptamers, single stranded RNA or DNA that bind to target ligands with high affinity and specificity, have a wide range of applications, from therapeutics to biosensing, which makes biochemical characterization of the utmost ... -
Catalytic Potential and Ligand Binding Properties of the Malachite Green RNA Aptamer
(University of Waterloo, 2008-09-18)The malachite green aptamer was originally engineered for binding specificity to malachite green (MG). The environment inside the aptamer’s electronegative binding pocket was previously harnessed to catalyze an ester ... -
Exploring Ligand Structure and Thermodynamics of the Malachite Green RNA Aptamer
(University of Waterloo, 2012-01-20)RNA aptamers are in vitro sequences of RNA that have a high affinity for their target ligand. They have applications in therapeutics, biosensors and molecular machines. While the practical applications of aptamers are ... -
Investigations into Streptomyces azureus Thiostrepton-resistance rRNA Methyltransferase and its Cognate Antibiotic
(University of Waterloo, 2008-12-16)Thiostrepton (TS: TS; C72H85N19O18S5) is a thiazoline antibiotic that is effective against Gram-positive bacteria and the malarial parasite, Plasmodium falciparum. Tight binding of TS to the bacterial L11-23S ribosomal RNA ... -
Metatranscriptomic analysis of pediatric acute sinusitis: pathogen detection and host response profiling
(University of Waterloo, 2024-05-08)Acute sinusitis (AS) is the fifth leading cause of antibiotic prescriptions in children. Distinguishing bacterial AS from common viral upper respiratory infections in children is crucial to prevent unnecessary antibiotic ... -
Preparation of a Multi-Part Varkud Satellite Ribozyme Variant for Kinetics Studies
(University of Waterloo, 2009-09-22)The Varkud Satellite (VS) ribozyme is the largest of the “small” nucleolytic ribozymes and is the only one for which there are no high resolution crystal structures available. The VS ribozyme comprises a catalytic domain ... -
RNA Homology Searches Using Pair Seeding
(University of Waterloo, 2005)Due to increasing numbers of non-coding RNA (ncRNA) being discovered recently, there is interest in identifying homologs of a given structured RNA sequence. Exhaustive homology searching for structured RNA molecules using ... -
Studying the Structure and Kinetics of Functional Nucleic Acids
(University of Waterloo, 2021-09-30)Aptamers are valuable tools in a variety of biotechnological applications, ranging from biosensing to in vivo cell imaging. Studying these molecules can further our understanding of the structure and kinetics of nucleic ...