Browsing Science (Faculty of) by Subject "DNA replication"
Now showing items 1-5 of 5
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The characterization of Dbf4 interactions and roles in genome replication and stability in Saccharomyces cerevisiae
(University of Waterloo, 2020-03-05)Dbf4-dependent kinase (DDK) is a complex composed of the Cdc7 kinase and its regulatory subunit, Dbf4. Initiation of eukaryotic DNA replication requires phosphorylation of Mcm2-7 helicase by DDK. Due to its essential role ... -
Characterization of Mrc1 during Replication Initiation in Saccharomyces cerevisiae
(University of Waterloo, 2022-01-12)DNA replication is a highly specific process that is completed under the control of several factors, to ensure faithful duplication of the genome. Mrc1 (Claspin in mammalian cells) is a protein that is a part of a fork ... -
Characterization of Orc6 function following pre-replicative complex assembly in Saccharomyces cerevisiae
(University of Waterloo, 2008-10-22)Pre-replicative complex (pre-RC) components the origin recognition complex (ORC), Cdc6, and Cdt1, play key roles in the recruitment, and loading of the replicative helicase, the minichromosome maintenance complex (Mcm2-7), ... -
Characterization of the role of Orc6 in the cell cycle of the budding yeast <em>Saccharomyces cerevisiae</em>
(University of Waterloo, 2006)The heterohexameric origin recognition complex (ORC) acts as a scaffold for the G1 phase assembly of pre-replicative complexes. Only the Orc1-5 subunits are required for origin binding in budding yeast, yet Orc6 is an ... -
A Quantitative Model of the Initiation of DNA Replication in Saccharomyces cerevisiae
(University of Waterloo, 2012-05-01)A crucial step in eukaryotic cell proliferation is the initiation of DNA replication, a tightly regulated process mediated by a multitude of protein factors. In Saccharomyces cerevisiae, this occurs as a result of the ...