A Cleavable Molecular Beacon for Hg2+ Detection based on Phosphorothioate RNA Modifications
| dc.contributor.author | Huang, Po-Jung Jimmy | |
| dc.contributor.author | Wang, Feng | |
| dc.contributor.author | Liu, Juewen | |
| dc.date.accessioned | 2017-02-23T20:53:48Z | |
| dc.date.available | 2017-02-23T20:53:48Z | |
| dc.date.issued | 2015-06-10 | |
| dc.description | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see http://dx.doi.org/ 10.1021/acs.analchem.5b01362 | en |
| dc.description.abstract | Mercury is a highly toxic heavy metal, and detection of Hg2+ by biosensors has attracted extensive research interest in the past decade. In particular, a number of DNA-based sensing strategies have been developed. Well-known examples include thymine-Hg2+ interactions and Hg2+-activated DNAzymes. However, these mechanisms are highly dependent on buffer conditions or require hybridization with another DNA strand. Herein, we report a new mechanism based on Hg2+-induced cleavage of phosphorothioate (PS) modified RNA. Among the various metal ions tested, Hg2+ induced the most significant cleavage (∼16%), while other metals cleaved less than 2% of the same substrate. The uncleaved substrate undergoes desulfurization in the presence of Hg2+. This cleavage reaction yields a similar amount of product from pH 3.5 to 7 and in the temperature range between 20 and 90 °C. Various PS RNA junctions can be cleaved with a similar efficiency, but PS DNA junctions cannot be cleaved. A molecular beacon containing three PS RNA modifications is designed, detecting Hg2+ down to 1.7 nM with excellent selectivity. This sensor can also detect Hg2+ in the Lake Ontario water sample, although its response is significantly masked by fish tissues. | en |
| dc.description.sponsorship | Ontario Ministry of Research & Innovation || Natural Sciences and Engineering Research Council || | en |
| dc.identifier.uri | http://dx.doi.org/10.1021/acs.analchem.5b01362 | |
| dc.identifier.uri | http://hdl.handle.net/10012/11345 | |
| dc.language.iso | en | en |
| dc.publisher | American Chemical Society | en |
| dc.subject | RNA | en |
| dc.subject | Cleavage | en |
| dc.subject | Mercury | en |
| dc.subject | Phosphorothioate | en |
| dc.title | A Cleavable Molecular Beacon for Hg2+ Detection based on Phosphorothioate RNA Modifications | en |
| dc.type | Article | en |
| dcterms.bibliographicCitation | Huang, P.-J. J., Wang, F., & Liu, J. (2015). Cleavable Molecular Beacon for Hg 2+ Detection Based on Phosphorothioate RNA Modifications. Analytical Chemistry, 87(13), 6890–6895. https://doi.org/10.1021/acs.analchem.5b01362 | en |
| uws.contributor.affiliation1 | Faculty of Science | en |
| uws.contributor.affiliation2 | Chemistry | en |
| uws.contributor.affiliation2 | Waterloo Institute for Nanotechnology (WIN) | en |
| uws.peerReviewStatus | Reviewed | en |
| uws.scholarLevel | Faculty | en |
| uws.typeOfResource | Text | en |