Unexpected high digestion rate of cooked starch by the Ct-Maltase-Glucoamylase small intenstine mucosal a-Glucosidase subunit
| dc.contributor.author | Lin, Amy Hui-Mei | |
| dc.contributor.author | Nichols, Buford L. | |
| dc.contributor.author | Quezada-Calvillo, Roberto | |
| dc.contributor.author | Avery, Stephen E. | |
| dc.contributor.author | Sim, Lyann | |
| dc.contributor.author | Rose, David R. | |
| dc.contributor.author | Naim, Hassam Y. | |
| dc.contributor.author | Hamaker, Bruce R. | |
| dc.date.accessioned | 2025-07-24T19:06:55Z | |
| dc.date.available | 2025-07-24T19:06:55Z | |
| dc.date.issued | 2012 | |
| dc.description | © 2012 Lin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
| dc.description.abstract | For starch digestion to glucose, two luminal a-amylases and four gut mucosal a-glucosidase subunits are employed. The aim of this research was to investigate, for the first time, direct digestion capability of individual mucosal a-glucosidases on cooked (gelatinized) starch. Gelatinized normal maize starch was digested with N- and C-terminal subunits of recombinant mammalian maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI) of varying amounts and digestion periods. Without the aid of a-amylase, Ct-MGAM demonstrated an unexpected rapid and high digestion degree near 80%, while other subunits showed 20 to 30% digestion. These findings suggest that Ct-MGAM assists a-amylase in digesting starch molecules and potentially may compensate for developmental or pathological amylase deficiencies. | |
| dc.description.sponsorship | Whistler Center for Carbohydrate Research at Purdue University || USDA, Agricultural Research Service, Cooperative Agreement no.58-6250-1-003. | |
| dc.identifier.uri | https://doi.org/10.1371/journal.pone.0035473 | |
| dc.identifier.uri | https://hdl.handle.net/10012/22052 | |
| dc.language.iso | en | |
| dc.publisher | Public Library of Science (PLOS) | |
| dc.relation.ispartofseries | PLOS One; 7(5) | |
| dc.rights | Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | starches | |
| dc.subject | glucose | |
| dc.subject | digestion | |
| dc.subject | maize | |
| dc.subject | hydrolysis | |
| dc.subject | diet | |
| dc.subject | glucans | |
| dc.subject | protein sequencing | |
| dc.title | Unexpected high digestion rate of cooked starch by the Ct-Maltase-Glucoamylase small intenstine mucosal a-Glucosidase subunit | |
| dc.type | Article | |
| dcterms.bibliographicCitation | Lin, A. H.-M., Nichols, B. L., Quezada-Calvillo, R., Avery, S. E., Sim, L., Rose, D. R., Naim, H. Y., & Hamaker, B. R. (2012). Unexpected high digestion rate of cooked starch by the CT-maltase-glucoamylase small intestine mucosal α-glucosidase subunit. PLoS ONE, 7(5). https://doi.org/10.1371/journal.pone.0035473 | |
| uws.contributor.affiliation1 | Faculty of Science | |
| uws.contributor.affiliation2 | Biology | |
| uws.peerReviewStatus | Reviewed | |
| uws.scholarLevel | Faculty | |
| uws.typeOfResource | Text | en |