Unexpected high digestion rate of cooked starch by the Ct-Maltase-Glucoamylase small intenstine mucosal a-Glucosidase subunit
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Date
2012
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Public Library of Science (PLOS)
Abstract
For starch digestion to glucose, two luminal a-amylases and four gut mucosal a-glucosidase subunits are employed. The aim of this research was to investigate, for the first time, direct digestion capability of individual mucosal a-glucosidases on cooked (gelatinized) starch. Gelatinized normal maize starch was digested with N- and C-terminal subunits of recombinant mammalian maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI) of varying amounts and digestion periods. Without the aid of a-amylase, Ct-MGAM demonstrated an unexpected rapid and high digestion degree near 80%, while other subunits showed 20 to 30% digestion. These findings suggest that Ct-MGAM assists a-amylase in digesting starch molecules and potentially may compensate for developmental or pathological amylase deficiencies.
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© 2012 Lin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords
starches, glucose, digestion, maize, hydrolysis, diet, glucans, protein sequencing