Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET

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dc.contributor.authorMuraih, Jawad K.
dc.contributor.authorPalmer, Michael
dc.date.accessioned2017-04-13T13:32:09Z
dc.date.available2017-04-13T13:32:09Z
dc.date.issued2012-07
dc.descriptionThe final publication is available at Elsevier via http://doi.org/10.1016/j.bbamem.2012.02.019 © 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.description.abstractDaptomycin is a lipopeptide antibiotic that kills Gram-positive bacteria by depolarizing their cell membranes. This antibacterial action of daptomycin is correlated with the formation of membrane-associated oligomers. We here examine the number of subunits contained in one oligomer using fluorescence resonance energy transfer (FRET). The results suggest that the oligomer contains approximately 6 to 7 subunits, or possibly twice this number if it spans both membrane monolayers.en
dc.description.sponsorshipThis work was supported by an NSERC operating grant to M. Palmer. J. Muraih was supported by a scholarship from the government of Iraq.en
dc.identifier.urihttps://doi.org/10.1016/j.bbamem.2012.02.019
dc.identifier.urihttp://hdl.handle.net/10012/11658
dc.language.isoenen
dc.publisherElsevieren
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAntibiotic daptomycinen
dc.subjectBactericidal activityen
dc.subjectBindingen
dc.subjectDaptomycinen
dc.subjectEnergy-transferen
dc.subjectFluorescence resonance energy transferen
dc.subjectLipopeptide antibioticsen
dc.subjectOligomer stoichiometryen
dc.subjectStaphylococcus-aureusen
dc.titleEstimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRETen
dc.typeArticleen
dcterms.bibliographicCitationMuraih, J. K., & Palmer, M. (2012). Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET. Biochimica Et Biophysica Acta-Biomembranes, 1818(7), 1642–1647. https://doi.org/10.1016/j.bbamem.2012.02.019en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen
uws.typeOfResourceTexten

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