Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET
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Date
2012-07
Authors
Muraih, Jawad K.
Palmer, Michael
Advisor
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Daptomycin is a lipopeptide antibiotic that kills Gram-positive bacteria by depolarizing their cell membranes. This antibacterial action of daptomycin is correlated with the formation of membrane-associated oligomers. We here examine the number of subunits contained in one oligomer using fluorescence resonance energy transfer (FRET). The results suggest that the oligomer contains approximately 6 to 7 subunits, or possibly twice this number if it spans both membrane monolayers.
Description
The final publication is available at Elsevier via http://doi.org/10.1016/j.bbamem.2012.02.019 © 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords
Antibiotic daptomycin, Bactericidal activity, Binding, Daptomycin, Energy-transfer, Fluorescence resonance energy transfer, Lipopeptide antibiotics, Oligomer stoichiometry, Staphylococcus-aureus