Browsing Science (Faculty of) by Subject "Lead"
Now showing items 1-5 of 5
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Distinction of Individual Lanthanide Ions with a DNAzyme Beacon Array
(American Chemical Society, 2016-06-24)Developing chemical probes to distinguish each lanthanide ion is a long-standing challenge. Aside from its analytical applications, solving this problem will also enhance our knowledge in metal ligand design. Using in vitro ... -
In Vitro Selection in Serum: RNA-Cleaving DNAzymes for Measuring Ca2+ and Mg2+
(American Chemical Society, 2016-05-27)RNA-cleaving DNAzymes have been attempted as in vivo analytical probes and gene silencing reagents over the past two decades. Despite progress already achieved, concerns still exist regarding the activity of DNAzymes in ... -
In Vitro Selection of a DNAzyme Cooperatively Binding Two Lanthanide Ions for RNA Cleavage
(American Chemical Society, 2016-05-03)Trivalent lanthanide ions (Ln(3+)) were recently employed to select RNA-cleaving DNAzymes, and three new DNAzymes have been reported so far. In this work, dysprosium (Dy3+) was used with a library containing 50 random ... -
Searching for a DNAzyme version of the leadzyme
(Springer, 2015-10-12)The leadzyme refers to a small ribozyme that cleaves a RNA substrate in the presence of Pb2+. In an optimized form, the enzyme strand contains only two unpaired nucleotides. Most RNA-cleaving DNAzymes are much longer. Two ... -
Two Pb2+-specific DNAzymes with opposite trends in split-site-dependent activity
(Royal Society of Chemistry, 2014-05-04)By splitting the catalytic core of DNAzymes into two halves, two Pb2+-specific DNAzymes retain partial activity, while they show opposite trends of activity as a function of the split site, revealing important nucleotides ...