Browsing Science (Faculty of) by Subject "Biosensor"
Now showing items 1-11 of 11
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Aptamer-based biosensors for biomedical diagnostics
(Royal Society of Chemistry, 2014-06-07)Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most aptamers are obtained through a combinatorial biology technique called SELEX. Since aptamers can be isolated to bind to almost any ... -
Co-immobilization of multiple enzymes by metal coordinated nucleotide hydrogel nanofibers: improved stability and an enzyme cascade for glucose detection
(Royal Society of Chemistry, 2016-03-21)Preserving enzyme activity and promoting synergistic activity via co-localization of multiple enzymes are key topics in bionanotechnology, materials science, and analytical chemistry. This study reports a facile method for ... -
Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing
(American Chemical Society, 2016-10-18)Fluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established sensing platform for detecting a diverse range of analytes. GO is a loosely defined material and its oxygen content may vary depending on the ... -
Distinction of Individual Lanthanide Ions with a DNAzyme Beacon Array
(American Chemical Society, 2016-06-24)Developing chemical probes to distinguish each lanthanide ion is a long-standing challenge. Aside from its analytical applications, solving this problem will also enhance our knowledge in metal ligand design. Using in vitro ... -
DNA stabilized fluorescent metal nanoclusters for biosensor development
(Elsevier, 2014-03-22)Fluorescent silver, gold and copper nanoclusters (NCs) have emerged for biosensor development. Compared to semiconductor quantum dots, there is less concern about the toxicity of metal NCs, which can be more easily conjugated ... -
In Vitro Selection in Serum: RNA-Cleaving DNAzymes for Measuring Ca2+ and Mg2+
(American Chemical Society, 2016-05-27)RNA-cleaving DNAzymes have been attempted as in vivo analytical probes and gene silencing reagents over the past two decades. Despite progress already achieved, concerns still exist regarding the activity of DNAzymes in ... -
In Vitro Selection of Chromium-Dependent DNAzymes for Sensing Chromium(III) and Chromium(VI)
(Wiley, 2016-07-04)Chromium is a very important analyte for environmental monitoring, and developing biosensors for chromium is a long-standing analytical challenge. In this work, in vitro selection of RNA-cleaving DNAzymes was carried out ... -
In Vitro Selection of New DNAzymes as Metal Biosensors
(University of Waterloo, 2015-06-26)In vitro selection of metal-specific DNAzymes has been shown to be a powerful method to obtain biosensors for metal ion detection. With 14 independent in vitro selection experiments using each of the trivalent lanthanide ... -
In-vitro selection, characterization and application of a silver-specific DNAzyme
(University of Waterloo, 2019-01-07)Evolution of novel DNA-based catalysts (DNAzymes) through in-vitro selection has expedited the research by manifolds, in both fundamental and analytical aspects of bio-nanotechnology. DNAzymes are attractive for their high ... -
A Silver DNAzyme
(American Chemical Society, 2016-04-05)Silver is a very common heavy metal, and its detection is of significant analytical importance. DNAzymes are DNA-based catalysts; they typically recruit divalent and trivalent metal ions for catalysis. Herein, we report a ... -
Towards point-of-care detection of polymicrobial infections: Rapid colorimetric response using a portable spectrophotometer
(Elsevier, 2016-09)Infectious diseases spread rapidly because current diagnostic methods are slow, expensive, and require technical expertise. Biosensors have recently been used as devices that can be deployed at the point-of-care for rapid ...