Browsing Science (Faculty of) by Subject "Aptamer"
Now showing items 1-7 of 7
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Aptamer-based biosensors for biomedical diagnostics
(Royal Society of Chemistry, 2014-06-07)Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most aptamers are obtained through a combinatorial biology technique called SELEX. Since aptamers can be isolated to bind to almost any ... -
Catalytic Potential and Ligand Binding Properties of the Malachite Green RNA Aptamer
(University of Waterloo, 2008-09-18)The malachite green aptamer was originally engineered for binding specificity to malachite green (MG). The environment inside the aptamer’s electronegative binding pocket was previously harnessed to catalyze an ester ... -
DNA and Hydrogels for Sensing and Binding Purine Nucleosides
(University of Waterloo, 2021-08-27)Purine nucleosides, such as adenosine and guanosine, are important biomolecules to regulate physiological functions, including maintain heart and brain health, exert inflammatory responses, and take part in metabolisms. ... -
DNA stabilized fluorescent metal nanoclusters for biosensor development
(Elsevier, 2014-03-22)Fluorescent silver, gold and copper nanoclusters (NCs) have emerged for biosensor development. Compared to semiconductor quantum dots, there is less concern about the toxicity of metal NCs, which can be more easily conjugated ... -
Exploring Ligand Structure and Thermodynamics of the Malachite Green RNA Aptamer
(University of Waterloo, 2012-01-20)RNA aptamers are in vitro sequences of RNA that have a high affinity for their target ligand. They have applications in therapeutics, biosensors and molecular machines. While the practical applications of aptamers are ... -
G-Quadruplex DNA for Fluorescent and Colorimetric Detection of Thallium(I)
(American Chemical Society, 2016-02-26)Thallium is a highly toxic heavy metal, but its 495 nm FRET sensing is underexplored compared to its neighboring elements Blue in the periodic table: lead and mercury. Thallium has two " oxidation states. A DNAzyme-based ... -
Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement
(American Chemical Society, 2014-12-16)Fluorescent aptamer probes physisorbed on graphene oxide (GO) have recently emerged as a useful sensing platform. A signal is generated by analyte-induced probe desorption. To address nonspecific probe displacement and the ...