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dc.contributor.authorJaroch, Karol
dc.contributor.authorPawliszyn, Janusz 21:37:45 (GMT) 21:37:45 (GMT)
dc.description.abstractThe solid-phase microextraction technique quantifies analytes without considerably affecting the sample composition. Herein, a proof-of-concept study was conducted to demonstrate the use of coated probe electrospray ionization (coated-PESI) and coated blade spray (CBS) as ambient mass spectrometry approaches for monitoring drug biotransformation. The ability of these methods was investigated for monitoring the dephosphorylation of a prodrug, combretastatin A4 phosphate (CA4P), into its active form, combretastatin A4 (CA4), in a cell culture medium supplemented with fetal bovine serum. The CBS spot analysis was modified to achieve the same extraction efficiency as protein precipitation and obtained results in 7 min. Because coated-PESI performs extraction without consuming any samples, it is the preferred technique in the case of a limited sample volume. Although coated-PESI only extracts small quantities of analytes, it uses the desorption solvent volume of 5–10 pL, resulting in high sensitivity, thus allowing the detection of compounds after only 1 min of extraction. The biotransformation of CA4P into CA4 via phosphatases occurs within the simple matrix, and the proposed sample preparation techniques are suitable for monitoring the biotransformation.en
dc.description.sponsorshipCanada's National Science and Engineering Research Council-Industrial Research Chair Program, Grant IRCPJ 184412-15.en
dc.relation.ispartofseriesJournal of Pharmaceutical Analysis;
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectsolid-phase microextractionen
dc.subjectcoated probe electrospray ionizationen
dc.subjectcoated blade sprayen
dc.subjectprodrug activationen
dc.titleTime-course monitoring of in vitro biotransformation reaction via solid-phase microextraction-ambient mass spectrometry approachesen
dcterms.bibliographicCitationJaroch, K., & Pawliszyn, J. (2021). Time-course monitoring of in vitro biotransformation reaction via solid-phase microextraction-ambient mass spectrometry approaches. Journal of Pharmaceutical Analysis.
uws.contributor.affiliation1Faculty of Scienceen

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Attribution-NonCommercial-NoDerivatives 4.0 International
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