Time-course monitoring of in vitro biotransformation reaction via solid-phase microextraction-ambient mass spectrometry approaches
| dc.contributor.author | Jaroch, Karol | |
| dc.contributor.author | Pawliszyn, Janusz | |
| dc.date.accessioned | 2022-02-03T21:37:45Z | |
| dc.date.available | 2022-02-03T21:37:45Z | |
| dc.date.issued | 2021-08 | |
| dc.description.abstract | The solid-phase microextraction technique quantifies analytes without considerably affecting the sample composition. Herein, a proof-of-concept study was conducted to demonstrate the use of coated probe electrospray ionization (coated-PESI) and coated blade spray (CBS) as ambient mass spectrometry approaches for monitoring drug biotransformation. The ability of these methods was investigated for monitoring the dephosphorylation of a prodrug, combretastatin A4 phosphate (CA4P), into its active form, combretastatin A4 (CA4), in a cell culture medium supplemented with fetal bovine serum. The CBS spot analysis was modified to achieve the same extraction efficiency as protein precipitation and obtained results in 7 min. Because coated-PESI performs extraction without consuming any samples, it is the preferred technique in the case of a limited sample volume. Although coated-PESI only extracts small quantities of analytes, it uses the desorption solvent volume of 5–10 pL, resulting in high sensitivity, thus allowing the detection of compounds after only 1 min of extraction. The biotransformation of CA4P into CA4 via phosphatases occurs within the simple matrix, and the proposed sample preparation techniques are suitable for monitoring the biotransformation. | en |
| dc.description.sponsorship | Canada's National Science and Engineering Research Council-Industrial Research Chair Program, Grant IRCPJ 184412-15. | en |
| dc.identifier.uri | https://doi.org/10.1016/j.jpha.2021.08.001 | |
| dc.identifier.uri | http://hdl.handle.net/10012/18045 | |
| dc.language.iso | en | en |
| dc.publisher | Elsevier | en |
| dc.relation.ispartofseries | Journal of Pharmaceutical Analysis; | |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.subject | solid-phase microextraction | en |
| dc.subject | coated probe electrospray ionization | en |
| dc.subject | coated blade spray | en |
| dc.subject | combretastatin | en |
| dc.subject | biotransformation | en |
| dc.subject | prodrug activation | en |
| dc.title | Time-course monitoring of in vitro biotransformation reaction via solid-phase microextraction-ambient mass spectrometry approaches | en |
| dc.type | Article | en |
| dcterms.bibliographicCitation | Jaroch, K., & Pawliszyn, J. (2021). Time-course monitoring of in vitro biotransformation reaction via solid-phase microextraction-ambient mass spectrometry approaches. Journal of Pharmaceutical Analysis. https://doi.org/10.1016/j.jpha.2021.08.001 | en |
| uws.contributor.affiliation1 | Faculty of Science | en |
| uws.contributor.affiliation2 | Chemistry | en |
| uws.peerReviewStatus | Reviewed | en |
| uws.scholarLevel | Faculty | en |
| uws.typeOfResource | Text | en |
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