Microparticle suspensions and bacteria laden droplets: Are they the same in terms of wetting signature?
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Date
2021-01-17
Authors
Melayil, Kiran Raj
Misra, Sirshendu
Mitra, Sushanta K.
Journal Title
Journal ISSN
Volume Title
Publisher
ACS
Abstract
Adhesion behavior of microbial pathogens on commonly encountered surfaces is one of the most pertinent questions now. We present the characterization of bacteria laden droplets and quantify the adhesion forces on highly repellent surfaces with the help of a simple experimental setup. Comparing the force signature measured directly using an in-house capillary deflection based droplet force apparatus, we report an anomalous adhesion behavior of live bacteria (E. coli) laden droplets on repellent surfaces, which stands in stark contrast to the observed adhesion signature when the doping agent is changed to inert microparticles or the same bacteria in an incapacitated state. We showed that the regular contact angle measurements using optical goniometry is unable to differentiate between the live bacteria and the dead ones (including microparticles) and thus delineate its limitations and the complementary nature of the adhesion measurements in understanding the fundamental interfacial interaction of living organisms on solid surfaces.
Description
This document is the Accepted Manuscript version of a Published Work that appeared in final form in LANGMUIR, copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see https://doi.org/10.1021/acs.langmuir.0c03365.
Keywords
microparticle, adhesion, wetting, bacteria, E. coli