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dc.contributor.authorZhou, Tong
dc.contributor.authorSong, Yongxin
dc.contributor.authorYuan, Yapeng
dc.contributor.authorLi, Dongqing
dc.date.accessioned2020-01-06 17:07:33 (GMT)
dc.date.available2020-01-06 17:07:33 (GMT)
dc.date.issued2019-04-04
dc.identifier.urihttps://doi.org/10.1016/j.aca.2018.11.049
dc.identifier.urihttp://hdl.handle.net/10012/15406
dc.descriptionThe final publication is available at Elsevier via https://doi.org/10.1016/j.aca.2018.11.049. © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.description.abstractTraditionally, a resistive pulse sensor (also known as Coulter counter) works by letting a particle pass through a small orifice in an electrolyte solution. The detection sensitivity mainly relies on the volume ratio of the particle to the orifice. This paper presents a novel resistive pulse sensor which has a sensing orifice located on the side wall of a microchannel. In this way, the sensor can detect and count particles (or cells) without requiring particles (or cells) passing through the sensing gate. An equation was derived to relate the magnitudes of the detected signals and the electrical resistances. Results show that the magnitudes of the detected signals can be increased by applying voltages from more than one voltage input channels simultaneously. Under the same conditions, the magnitudes of the detected signals become larger when the diameters of particles are larger. Higher detection sensitivity can be obtained simply by increasing either the magnitudes of the applied voltages or the number of the voltage input channels, or reducing the opening of the side sensing gate to a size that is even smaller than the diameter of the particle. Due to the high detection sensitivity, detection of 1 μm particles by a relatively large sensing gate of 5 × 10 × 10 μm (width × length × height) was successfully demonstrated with a signal to noise ratio (S/N) of approximately 3. This sensor was also applied to detect and count human red blood cells and lymphocyte cells. Results show that this method can clearly distinguish the cells with different sizes based on the pre-determined-thresholds. Because this sensor does not require cells to pass through the sensing gate, the channel clogging problem can be avoided. More importantly, the detection sensitivity can be tuned by applying different voltages without fabricating a smaller sensing gate.en
dc.description.sponsorshipThe authors wish to acknowledge financial support of National Natural Science Foundation program of China (51679023) and Liaoning BaiQianWan Talents Program to Yongxin Song; the Natural Sciences and Engineering Research Council of Canada through a research grant to D. Li, the support from Fundamental Research Funds for the Central Universities (3132016325) and from the University 111 project of China under Grant No. B08046 is greatly appreciated.en
dc.language.isoenen
dc.publisherElsevieren
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectresistive pulse sensingen
dc.subjectmultiple voltage input channelsen
dc.subjectside sensing gateen
dc.subjectcell detectionen
dc.titleA novel microfluidic resistive pulse sensor with multiple voltage input channels and a side sensing gate for particle and cell detectionen
dc.typeArticleen
dcterms.bibliographicCitationT. Zhou, Y. Song, Y. Yuan, D. Li, A Novel Microfluidic Resistive Pulse Sensor with Multiple Voltage Input Channels and a Side Sensing Gate for Particle and Cell Detection, Analytica Chimica Acta, https://doi.org/10.1016/j.aca.2018.11.049.en
uws.contributor.affiliation1Faculty of Engineeringen
uws.contributor.affiliation2Mechanical and Mechatronics Engineeringen
uws.typeOfResourceTexten
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen


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