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dc.contributor.authorLiang, Hao
dc.contributor.authorJiang, Shuhui
dc.contributor.authorYuan, Qipeng
dc.contributor.authorLi, Guofeng
dc.contributor.authorWang, Feng
dc.contributor.authorZhang, Zijie
dc.contributor.authorLiu, Juewen
dc.date.accessioned2017-04-28 16:11:59 (GMT)
dc.date.available2017-04-28 16:11:59 (GMT)
dc.date.issued2016-03-21
dc.identifier.urihttp://dx.doi.org/10.1039/c5nr08734a
dc.identifier.urihttp://hdl.handle.net/10012/11796
dc.description.abstractPreserving enzyme activity and promoting synergistic activity via co-localization of multiple enzymes are key topics in bionanotechnology, materials science, and analytical chemistry. This study reports a facile method for co-immobilizing multiple enzymes in metal coordinated hydrogel nanofibers. Specifically, four types of protein enzymes, including glucose oxidase, Candida rugosa lipase, a-amylase, and horseradish peroxidase, were respectively encapsulated in a gel nanofiber made of Zn2+ and adenosine monophosphate (AMP) with a simple mixing step. Most enzymes achieved quantitative loading and retained full activity. At the same time, the entrapped enzymes were more stable against temperature variation (by 7.5 degrees C), protease attack, extreme pH (by 2-fold), and organic solvents. After storing for 15 days, the entrapped enzyme still retained 70% activity while the free enzyme nearly completely lost its activity. Compared to nanoparticles formed with AMP and lanthanide ions, the nanofiber gels allowed much higher enzyme activity. Finally, a highly sensitive and selective biosensor for glucose was prepared using the gel nanofiber to co-immobilize glucose oxidase and horseradish peroxidase for an enzyme cascade system. A detection limit of 0.3 mu M glucose with excellent selectivity was achieved. This work indicates that metal coordinated materials using nucleotides are highly useful for interfacing with biomolecules.en
dc.description.sponsorshipBeijing Higher Education Young Elite Teacher Project [YETP0520]; Fundamental Research Funds for the Central Universities [YS1407]; Beijing Natural Science Foundation [2162030]; China Scholarship Council; Natural Sciences and Engineering Research Council of Canada (NSERC)en
dc.language.isoenen
dc.publisherRoyal Society of Chemistryen
dc.subjectInorganic Hybrid Nanoflowersen
dc.subjectOrganic Frameworksen
dc.subjectImmobilizationen
dc.subjectNanoparticlesen
dc.subjectComplexesen
dc.subjectEncapsulationen
dc.subjectBiocatalysisen
dc.subjectNucleobaseen
dc.subjectScaffoldsen
dc.subjectBiosensoren
dc.titleCo-immobilization of multiple enzymes by metal coordinated nucleotide hydrogel nanofibers: improved stability and an enzyme cascade for glucose detectionen
dc.typeArticleen
dcterms.bibliographicCitationLiang, H., Jiang, S., Yuan, Q., Li, G., Wang, F., Zhang, Z., & Liu, J. (2016). Co-immobilization of multiple enzymes by metal coordinated nucleotide hydrogel nanofibers: improved stability and an enzyme cascade for glucose detection. Nanoscale, 8(11), 6071–6078. https://doi.org/10.1039/c5nr08734aen
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.contributor.affiliation3Waterloo Institute for Nanotechnology (WIN)en
uws.typeOfResourceTexten
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen


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