Attaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenching
MetadataShow full item record
Cerium oxide nanoparticles (nanoceria) have recently emerged as a nanozyme with oxidase activity. In this work, we present a few important interfacial properties of nanoceria. First, the surface charge of nanoceria can be controlled not only by adjusting pH but also by adsorption of simple inorganic anions. Adsorption of phosphate and citrate gives negatively charged surface over a broad pH range. Second, nanoceria adsorbs DNA via the DNA phosphate backbone in a sequence-independent manner; DNA adsorption inhibits its oxidase activity. Other anionic polymers display much weaker inhibition effects. Adsorption of simple inorganic phosphate does not have the inhibition effect. Third, nanoceria is a quencher for many fluorophores. These discoveries provide an important understanding for further use of nanoceria in biosensor development, materials science, and nanotechnology.
Cite this version of the work
Rachel Pautler, Erin Y. Kelly, Po-Jung Jimmy Huang, Jing Cao, Biwu Liu, Juewen Liu (2013). Attaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenching. UWSpace. http://hdl.handle.net/10012/11387