Attaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenching

dc.contributor.authorPautler, Rachel
dc.contributor.authorKelly, Erin Y.
dc.contributor.authorHuang, Po-Jung Jimmy
dc.contributor.authorCao, Jing
dc.contributor.authorLiu, Biwu
dc.contributor.authorLiu, Juewen
dc.date.accessioned2017-03-01T15:33:36Z
dc.date.available2017-03-01T15:33:36Z
dc.date.issued2013-08-14
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Applied Materials and Interfaces copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Pautler, R., Kelly, E. Y., Huang, P.-J. J., Cao, J., Liu, B., & Liu, J. (2013). Attaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenching. ACS Applied Materials & Interfaces, 5(15), 6820–6825. https://doi.org/10.1021/am4018863en
dc.description.abstractCerium oxide nanoparticles (nanoceria) have recently emerged as a nanozyme with oxidase activity. In this work, we present a few important interfacial properties of nanoceria. First, the surface charge of nanoceria can be controlled not only by adjusting pH but also by adsorption of simple inorganic anions. Adsorption of phosphate and citrate gives negatively charged surface over a broad pH range. Second, nanoceria adsorbs DNA via the DNA phosphate backbone in a sequence-independent manner; DNA adsorption inhibits its oxidase activity. Other anionic polymers display much weaker inhibition effects. Adsorption of simple inorganic phosphate does not have the inhibition effect. Third, nanoceria is a quencher for many fluorophores. These discoveries provide an important understanding for further use of nanoceria in biosensor development, materials science, and nanotechnology.en
dc.description.sponsorshipUniversity of Waterloo || Canadian Foundation for Innovation || Natural Sciences and Engineering Research Council || Ontario Ministry of Research and Innovation ||en
dc.identifier.urihttp://dx.doi.org/10.1021/am4018863
dc.identifier.urihttp://hdl.handle.net/10012/11387
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.subjectcerium oxideen
dc.subjectnanozymesen
dc.subjectadsorptionen
dc.subjectsurface chargeen
dc.subjectoxidaseen
dc.titleAttaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenchingen
dc.typeArticleen
dcterms.bibliographicCitationPautler, R., Kelly, E. Y., Huang, P.-J. J., Cao, J., Liu, B., & Liu, J. (2013). Attaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenching. ACS Applied Materials & Interfaces, 5(15), 6820–6825. https://doi.org/10.1021/am4018863en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.contributor.affiliation2Waterloo Institute for Nanotechnology (WIN)en
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen
uws.typeOfResourceTexten

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