Open Port Probe Sampling Interface for the Direct Coupling of Biocompatible Solid-Phase Microextraction to Atmospheric Pressure Ionization Mass Spectrometry

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dc.contributor.authorGomez-Rios, German Augusto
dc.contributor.authorLiu, Chang
dc.contributor.authorTascon, Marcos
dc.contributor.authorReyes-Garcés, Nathaly
dc.contributor.authorArnold, Don W.
dc.contributor.authorCovey, Thomas R.
dc.contributor.authorPawliszyn, Janusz
dc.date.accessioned2017-09-11T15:50:46Z
dc.date.available2017-09-11T15:50:46Z
dc.date.issued2017-04-04
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.analchem.6b04737en
dc.description.abstractIn recent years, the direct coupling of solid phase microextraction (SPME) and mass spectrometry (MS) has shown its great potential to improve limits of quantitation, accelerate analysis throughput, and diminish potential matrix effects when compared to direct injection to MS. In this study, we introduce the open port probe (OPP) as a robust interface to couple biocompatible SPME (Bio-SPME) fibers to MS systems for direct electrospray ionization. The presented design consisted of minimal alterations to the front-end of the instrument and provided better sensitivity, simplicity, speed, wider compound coverage, and high-throughput in comparison to the LC-MS based approach. Quantitative determination of clenbuterol, fentanyl, and buprenorphine was successfully achieved in human urine. Despite the use of short extraction/desorption times (5 min/5 s), limits of quantitation below the minimum required performance levels (MRPL) set by the world antidoping agency (WADA) were obtained with good accuracy (>= 90%) and linearity (R-2 > 0.99) over the range evaluated for all analytes using sample volumes of 300 mu L. In-line technologies such as multiple reaction monitoring with multistage fragmentation (MRM3) and differential mobility spectrometry (DMS) were used to enhance the selectivity of the method without compromising analysis speed. On the basis of calculations, once coupled to high throughput, this method can potentially yield preparation times as low as 15 s per sample based on the 96-well plate format. Our results demonstrated that Bio-SPME-OPP-MS efficiently integrates sampling/sample cleanup and atmospheric pressure ionization, making it an advantageous configuration for several bioanalytical applications, including doping in sports, in vivo tissue sampling, and therapeutic drug monitoring.en
dc.description.sponsorshipSCIEXen
dc.description.sponsorshipNatural Sciences and Engineering Research Council (NSERC) of Canadaen
dc.identifier.urihttp://dx.doi.org/10.1021/acs.analchem.6b04737
dc.identifier.urihttp://hdl.handle.net/10012/12358
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.subjectProhibited Substancesen
dc.subjectAmbient Ionizationen
dc.subjectBiological Samplesen
dc.subjectComplex Matricesen
dc.subjectTarget Analytesen
dc.subjectIon Mobilityen
dc.subjectQuantitationen
dc.subjectElectrosprayen
dc.subjectBioanalysisen
dc.subjectDevicesen
dc.titleOpen Port Probe Sampling Interface for the Direct Coupling of Biocompatible Solid-Phase Microextraction to Atmospheric Pressure Ionization Mass Spectrometryen
dc.typeArticleen
dcterms.bibliographicCitationGómez-Ríos, G. A., Liu, C., Tascon, M., Reyes-Garcés, N., Arnold, D. W., Covey, T. R., & Pawliszyn, J. (2017). Open Port Probe Sampling Interface for the Direct Coupling of Biocompatible Solid-Phase Microextraction to Atmospheric Pressure Ionization Mass Spectrometry. Analytical Chemistry, 89(7), 3805–3809. https://doi.org/10.1021/acs.analchem.6b04737en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen
uws.typeOfResourceTexten

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