A Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescence
dc.contributor.author | Zhou, Wenhu | |
dc.contributor.author | Ding, Jinsong | |
dc.contributor.author | Liu, Juewen | |
dc.date.accessioned | 2017-04-28T16:12:06Z | |
dc.date.available | 2017-04-28T16:12:06Z | |
dc.date.issued | 2016-08-17 | |
dc.description | This is the peer reviewed version of the following article: Zhou, W., Ding, J., & Liu, J. (2016). A Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescence. Chembiochem, 17(16), 1563–1570. https://doi.org/10.1002/cbic.201600174, which has been published in final form at https://doi.org/10.1002/cbic.201600174. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | en |
dc.description.abstract | A previous study of two RNA-cleaving DNAzymes, NaA43 and Ce13d, revealed the possibility of a common Na+ aptamer motif. Because Na+ binding to DNA is a fundamental biochemical problem, the interaction between Ce13d and Na+ was studied in detail by using sensitized Tb3+ luminescence spectroscopy. Na+ displaces Tb3+ from the DNAzyme, and thus quenches the emission from Tb3+. The overall requirement for Na+ binding includes the hairpin and the highly conserved 16-nucleotide loop in the enzyme strand, along with a few unpaired nucleotides in the substrate. Mutation studies indicate good correlation between Na+ binding and cleavage activity, thus suggesting a critical role of Na+ binding for the enzyme activity. Ce13d displayed a K-d of approximate to 20mm with Na+ (other monovalent cations: 40-60mm). The K-d values for other metal ions are mainly due to non-specific competition. With a single nucleotide mutation, the specific Na+ binding was lost. Another mutant improved K-d to 8mm with Na+. This study has demonstrated a Na+ aptamer with important biological implications and analytical applications. It has also defined the structural requirements for Na+ binding and produced an improved mutant. | en |
dc.description.sponsorship | University of Waterloo; Natural Sciences and Engineering Research Council of Canada (NSERC); Foundation for Shenghua Scholar of Central South University; National Natural Science Foundation of China [21301195]; China Scholarship Council (CSC) [201406370116] | en |
dc.identifier.uri | http://dx.doi.org/10.1002/cbic.201600174 | |
dc.identifier.uri | http://hdl.handle.net/10012/11811 | |
dc.language.iso | en | en |
dc.publisher | Wiley | en |
dc.subject | In-Vitro Selection | en |
dc.subject | Lanthanide-Dependent DNAzyme | en |
dc.subject | Intracellular Sodium-Ions | en |
dc.subject | Metal-Ions | en |
dc.subject | Monovalent Cations | en |
dc.subject | DNA Duplexes | en |
dc.subject | Ribozyme | en |
dc.subject | Binding | en |
dc.subject | Sensor | en |
dc.subject | Acid | en |
dc.title | A Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescence | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Zhou, W., Ding, J., & Liu, J. (2016). A Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescence. Chembiochem, 17(16), 1563–1570. https://doi.org/10.1002/cbic.201600174 | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Chemistry | en |
uws.contributor.affiliation3 | Waterloo Institute for Nanotechnology (WIN) | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |
uws.typeOfResource | Text | en |
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