A Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescence

Simple item page
dc.contributor.authorZhou, Wenhu
dc.contributor.authorDing, Jinsong
dc.contributor.authorLiu, Juewen
dc.date.accessioned2017-04-28T16:12:06Z
dc.date.available2017-04-28T16:12:06Z
dc.date.issued2016-08-17
dc.descriptionThis is the peer reviewed version of the following article: Zhou, W., Ding, J., & Liu, J. (2016). A Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescence. Chembiochem, 17(16), 1563–1570. https://doi.org/10.1002/cbic.201600174, which has been published in final form at https://doi.org/10.1002/cbic.201600174. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.en
dc.description.abstractA previous study of two RNA-cleaving DNAzymes, NaA43 and Ce13d, revealed the possibility of a common Na+ aptamer motif. Because Na+ binding to DNA is a fundamental biochemical problem, the interaction between Ce13d and Na+ was studied in detail by using sensitized Tb3+ luminescence spectroscopy. Na+ displaces Tb3+ from the DNAzyme, and thus quenches the emission from Tb3+. The overall requirement for Na+ binding includes the hairpin and the highly conserved 16-nucleotide loop in the enzyme strand, along with a few unpaired nucleotides in the substrate. Mutation studies indicate good correlation between Na+ binding and cleavage activity, thus suggesting a critical role of Na+ binding for the enzyme activity. Ce13d displayed a K-d of approximate to 20mm with Na+ (other monovalent cations: 40-60mm). The K-d values for other metal ions are mainly due to non-specific competition. With a single nucleotide mutation, the specific Na+ binding was lost. Another mutant improved K-d to 8mm with Na+. This study has demonstrated a Na+ aptamer with important biological implications and analytical applications. It has also defined the structural requirements for Na+ binding and produced an improved mutant.en
dc.description.sponsorshipUniversity of Waterloo; Natural Sciences and Engineering Research Council of Canada (NSERC); Foundation for Shenghua Scholar of Central South University; National Natural Science Foundation of China [21301195]; China Scholarship Council (CSC) [201406370116]en
dc.identifier.urihttp://dx.doi.org/10.1002/cbic.201600174
dc.identifier.urihttp://hdl.handle.net/10012/11811
dc.language.isoenen
dc.publisherWileyen
dc.subjectIn-Vitro Selectionen
dc.subjectLanthanide-Dependent DNAzymeen
dc.subjectIntracellular Sodium-Ionsen
dc.subjectMetal-Ionsen
dc.subjectMonovalent Cationsen
dc.subjectDNA Duplexesen
dc.subjectRibozymeen
dc.subjectBindingen
dc.subjectSensoren
dc.subjectAciden
dc.titleA Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescenceen
dc.typeArticleen
dcterms.bibliographicCitationZhou, W., Ding, J., & Liu, J. (2016). A Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescence. Chembiochem, 17(16), 1563–1570. https://doi.org/10.1002/cbic.201600174en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.contributor.affiliation3Waterloo Institute for Nanotechnology (WIN)en
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen
uws.typeOfResourceTexten

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
A Selective Na+ Aptamer Dissected by Sensitized Tb3+ Luminescence-s.pdf
Size:
1.53 MB
Format:
Adobe Portable Document Format
Description:
post-print
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.46 KB
Format:
Plain Text
Description:
Download

Collections

Waterloo Research
Chemistry
Waterloo Institute for Nanotechnology