Characterization of daptomycin oligomerization with perylene excimer fluorescence: Stoichiometric binding of phosphatidylglycerol triggers oligomer formation

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dc.contributor.authorMuraih, Jawad K.
dc.contributor.authorHarris, Jesse
dc.contributor.authorTaylor, Scott D.
dc.contributor.authorPalmer, Michael
dc.date.accessioned2017-04-13T17:45:08Z
dc.date.available2017-04-13T17:45:08Z
dc.date.issued2012-03
dc.descriptionThe final publication is available at Elsevier via http://dx.doi.org/10.1016/j.bbamem.2011.10.027 © 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.description.abstractDaptomycin is a lipopeptide antibiotic that binds to and depolarizes bacterial cell membranes. Its antibacterial activity requires calcium and correlates with the content of phosphatidylglycerol in the target membrane. Daptomycin has been shown to form oligomers on liposome membranes. We here use perylene excimer fluorescence to further characterize the membrane-associated oligomer. To this end, the N-terminal fatty acyl chain was replaced with perylene-butanoic acid. The perylene derivative retains one third of the antibacterial activity of native daptomycin. On liposomes containing phosphatidylcholine and phosphatidylglycerol, as well as on Bacillus subtilis cells, the perylene-labeled daptomycin forms excimers, which shows that the N-terminal acyl chains of neighboring oligomer subunits are in immediate contact with one another. In a lipid bicelle system, oligomer formation can be titrated with stoichiometric amounts of phosphatidylglycerol. Therefore, the interaction of daptomycin with a single molecule of phosphatidylglycerol is sufficient to trigger daptomycin oligomerization.en
dc.description.sponsorshipNSERC operating grants to M. Palmer and S. D. Taylor. || J. Muraih was supported by a scholarship from the government of Iraqen
dc.identifier.urihttps://doi.org/10.1016/j.bbamem.2011.10.027
dc.identifier.urihttp://hdl.handle.net/10012/11664
dc.language.isoenen
dc.publisherElsevieren
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBacillus-subtilisen
dc.subjectBactericidal activityen
dc.subjectDaptomycinen
dc.subjectExcimer fluorescenceen
dc.subjectLeadsen
dc.subjectLipopeptideen
dc.subjectly146032en
dc.subjectMembranesen
dc.subjectOligomerizationen
dc.subjectPeryleneen
dc.subjectPhosphatidylglycerolen
dc.subjectStaphylococcus-aureusen
dc.titleCharacterization of daptomycin oligomerization with perylene excimer fluorescence: Stoichiometric binding of phosphatidylglycerol triggers oligomer formationen
dc.typeArticleen
dcterms.bibliographicCitationMuraih, J. K., Harris, J., Taylor, S. D., & Palmer, M. (2012). Characterization of daptomycin oligomerization with perylene excimer fluorescence: Stoichiometric binding of phosphatidylglycerol triggers oligomer formation. Biochimica Et Biophysica Acta-Biomembranes, 1818(3), 673–678. https://doi.org/10.1016/j.bbamem.2011.10.027en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen
uws.typeOfResourceTexten

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