Extracellular dsRNA induces a type I interferon response mediated via class A scavenger receptors in a novel Chinook salmon derived spleen cell line
dc.contributor.author | Semple, Shawna L. | |
dc.contributor.author | Vo, Nguyen T. K. | |
dc.contributor.author | Poynter, Sarah J. | |
dc.contributor.author | Li, M. | |
dc.contributor.author | Heath, Daniel D. | |
dc.contributor.author | DeWitte-Orr, Stephanie J. | |
dc.contributor.author | Dixon, Brian | |
dc.date.accessioned | 2018-11-14T16:43:24Z | |
dc.date.available | 2018-11-14T16:43:24Z | |
dc.date.issued | 2018-12-01 | |
dc.description | The final publication is available at Elsevier via https://dx.doi.org/10.1016/j.dci.2018.08.010 © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
dc.description.abstract | Despite increased global interest in Chinook salmon aquaculture, little is known of their viral immune defenses. This study describes the establishment and characterization of a continuous cell line derived from Chinook salmon spleen, CHSS, and its use in innate immune studies. Optimal growth was seen at 14–18 °C when grown in Leibovitz's L-15 media with 20% fetal bovine serum. DNA analyses confirmed that CHSS was Chinook salmon and genetically different from the only other available Chinook salmon cell line, CHSE-214. Unlike CHSE-214, CHSS could bind extracellular dsRNA, resulting in the rapid and robust expression of antiviral genes. Receptor/ligand blocking assays confirmed that class A scavenger receptors (SR-A) facilitated dsRNA binding and subsequent gene expression. Although both cell lines expressed three SR-A genes: SCARA3, SCARA4, and SCARA5, only CHSS appeared to have functional cell-surface SR-As for dsRNA. Collectively, CHSS is an excellent cell model to study dsRNA-mediated innate immunity in Chinook salmon. | en |
dc.description.sponsorship | Natural Sciences and Engineering Research Council of Canada | en |
dc.description.sponsorship | Canada Research Council | en |
dc.identifier.uri | https://dx.doi.org/10.1016/j.dci.2018.08.010 | |
dc.identifier.uri | http://hdl.handle.net/10012/14116 | |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cell line | en |
dc.subject | Chinook salmon | en |
dc.subject | dsRNA | en |
dc.subject | Innate immunity | en |
dc.subject | Interferon-stimulated genes | en |
dc.subject | polyinosinic:polycytidylic acid | en |
dc.title | Extracellular dsRNA induces a type I interferon response mediated via class A scavenger receptors in a novel Chinook salmon derived spleen cell line | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Semple, S. L., Vo, N. T. K., Poynter, S. J., Li, M., Heath, D. D., DeWitte-Orr, S. J., & Dixon, B. (2018). Extracellular dsRNA induces a type I interferon response mediated via class A scavenger receptors in a novel Chinook salmon derived spleen cell line. Developmental & Comparative Immunology, 89, 93–101. doi:10.1016/j.dci.2018.08.010 | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Biology | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |
uws.typeOfResource | Text | en |
uws.typeOfResource | Text | en |