Sensing Parts-per-Trillion Cd2+, Hg2+ and Pb2+ Collectively and Individually Using Phosphorothioate DNAzymes
| dc.contributor.author | Huang, Po-Jung Jimmy | |
| dc.contributor.author | Liu, Juewen | |
| dc.date.accessioned | 2017-02-27T20:23:57Z | |
| dc.date.available | 2017-02-27T20:23:57Z | |
| dc.date.issued | 2014-05-22 | |
| dc.description | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Huang, P.-J. J., & Liu, J. (2014). Sensing Parts-per-Trillion Cd 2+ , Hg 2+ , and Pb 2+ Collectively and Individually Using Phosphorothioate DNAzymes. Analytical Chemistry, 86(12), 5999–6005. https://doi.org/10.1021/ac501070a | en |
| dc.description.abstract | Cadmium, mercury, and lead are collectively banned by many countries and regions in electronic devices due to their extremely high toxicity. To date, no sensing method can detect them as a group and also individually with sufficient sensitivity and selectivity. An RNA-cleaving DNAzyme (Ce13d) was recently reported to be active with trivalent lanthanides, which are hard Lewis acids. In this work, phosphorothioate (PS) modifications were systematically made on Ce13d. A single PS modification at the substrate cleavage site shifts the activity from being dependent on lanthanides to soft thiophilic metals. By incorporating the PS modification to another DNAzyme, a sensor array was prepared to detect each metal. Individual sensors have excellent sensitivity (limit of detection = 4.8 nM Cd2+, 2.0 nM Hg2+, and 0.1 nM Pb2+). This study provides a new route to obtain metal-specific DNAzymes by atomic replacement and also offers important mechanistic insights into metal binding and DNAzyme catalysis. | en |
| dc.description.sponsorship | University of Waterloo || Ontario Ministry of Research & Innovation || Natural Sciences and Engineering Research Council || | en |
| dc.identifier.uri | http://dx.doi.org/10.1021/ac501070a | |
| dc.identifier.uri | http://hdl.handle.net/10012/11367 | |
| dc.language.iso | en | en |
| dc.publisher | American Chemical Society | en |
| dc.subject | DNAzymes | en |
| dc.subject | RNA cleavage | en |
| dc.subject | Metal ions | en |
| dc.title | Sensing Parts-per-Trillion Cd2+, Hg2+ and Pb2+ Collectively and Individually Using Phosphorothioate DNAzymes | en |
| dc.type | Article | en |
| dcterms.bibliographicCitation | Huang, P.-J. J., & Liu, J. (2014). Sensing Parts-per-Trillion Cd 2+ , Hg 2+ , and Pb 2+ Collectively and Individually Using Phosphorothioate DNAzymes. Analytical Chemistry, 86(12), 5999–6005. https://doi.org/10.1021/ac501070a | en |
| uws.contributor.affiliation1 | Faculty of Science | en |
| uws.contributor.affiliation2 | Chemistry | en |
| uws.contributor.affiliation2 | Waterloo Institute for Nanotechnology (WIN) | en |
| uws.peerReviewStatus | Reviewed | en |
| uws.scholarLevel | Faculty | en |
| uws.typeOfResource | Text | en |