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Cinnamaldehyde Characterization as an Antibacterial Agent toward E. coli Metabolic Profile Using 96-Blade Solid-Phase Microextraction Coupled to Liquid Chromatography–Mass Spectrometry

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dc.contributor.authorMousavi, Fatemeh
dc.contributor.authorBojko, Barbara
dc.contributor.authorBessonneau, Vincent
dc.contributor.authorPawliszyn, Janusz
dc.date.accessioned2016-10-27T15:38:36Z
dc.date.available2016-10-27T15:38:36Z
dc.date.issued2016-01-26
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Proteome Research, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.jproteome.5b00992en
dc.description.abstractSampling and sample preparation plays an important role in untargeted analysis as it influences final composition of the analyzed extract and consequently reflection of the metabolome. In the current work, mechanism of bactericidal action of cinnamaldehyde (CA) against Escherichia coli (E. coli) during bacteria growth applying high-throughput solid-phase microextraction in direct immersion mode coupled to a high-performance liquid chromatography–mass spectrometry system was investigated. Numerous discriminant metabolites due to CA addition to the bacteria culture were mapped in the E. coli metabolic pathways. We propose new metabolic pathways confirming that CA acts as an oxidative stress agent against E. coli. The results of the current research have successfully demonstrated that CA changes the bacterial metabolism through interactions with different biochemical families such as proteins, nucleic acids, lipids, and carbohydrates, which needs further validation by proteomics and transcriptomics studies. The results presented here show the great potential of the novel approach in drug discovery and food safety.en
dc.description.sponsorshipNatural Sciences and Engineering Research Council (NSERC) of Canadaen
dc.identifier.urihttp://dx.doi.org/10.1021/acs.jproteome.5b00992
dc.identifier.urihttp://hdl.handle.net/10012/11036
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.titleCinnamaldehyde Characterization as an Antibacterial Agent toward E. coli Metabolic Profile Using 96-Blade Solid-Phase Microextraction Coupled to Liquid Chromatography–Mass Spectrometryen
dc.typeArticleen
dcterms.bibliographicCitationMousavi, F., Bojko, B., Bessonneau, V., & Pawliszyn, J. (2016). Cinnamaldehyde Characterization as an Antibacterial Agent toward E. coli Metabolic Profile Using 96-Blade Solid-Phase Microextraction Coupled to Liquid Chromatography–Mass Spectrometry. Journal of Proteome Research, 15(3), 963–975. https://doi.org/10.1021/acs.jproteome.5b00992en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen
uws.typeOfResourceTexten

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