DNA stabilized silver nanoclusters for ratiometric and visual detection of Hg2+ and its immobilization in hydrogels
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Date
2013-04-11
Authors
MacLean, James L.
Morishita, Kiyoshi
Liu, Juewen
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
DNA oligomers are particularly interesting templates for making silver nanoclusters (AgNCs) as different emission colors can be obtained by varying the DNA sequence. Many AgNCs have been used as Hg2+ sensors since Hg2+ induces fluorescence quenching. From an analytical chemistry standpoint, however, these ‘light off’ sensors are undesirable. In this work, taking advantage of the fact that some AgNCs are not as effectively quenched by Hg2+, we design a sensor with AgNCs containing two emission peaks. The red peak is strongly quenched by Hg2+ while the green peak shows a concomitant increase, producing an orange-to-green visual fluorescence transformation. Using this AgNC, we demonstrate ratiometric detection with a detection limit of 4 nM Hg2+. This sensor is further immobilized in a hydrogel matrix and this gel is also capable of detecting Hg2+ with a visual response.
Description
The final publication is available at Elsevier via http://dx.doi.org/10.1016/j.bios.2013.04.002." © 2013. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords
mercury, silver nanoclusters, fluorescence, ratiometric, biosensors